tA study of the process of apoptosis by measurement of the caspase-8 activity in peripheral blood T lymphocytes in chronic hepatitis C infected patients
• Manal Said Fowzy;
Abstract
The present work aimed to identify apoptosis (by assay of
';
caspase-8 specific activity by ELTSA and determination of DNA
fragmentation by agarose gel electrophoresis) in PB lymphocytes derived from chronic HCV-patients and to detect some changes that might occur in their PB lymphocyte subsets (CD3+/4+/8+ and CDI6+/19+) by flowcytometery. Concerning caspase-8 specific activity, it was comparable in both groups, however, patients having DNA fragmentation
• (46.5%), showed statistically significant higher activity than those without detectable DNA fragmentation (p=0.03). All controls were negative for DNA fragmentation. In addition, a statistically significant positive correlation was found between absolute count of peripheral blood lymphocyte, CD3+, CDS+ and CD!9+ subpopulations of
.lymphocytes and plasma ALT level in patients with negativt• HCV-PCR. T:herefore, apoptosis is probably a major mechanism in pathogenesis of chronic HCV infection responsible for perpetuation of infection.
';
caspase-8 specific activity by ELTSA and determination of DNA
fragmentation by agarose gel electrophoresis) in PB lymphocytes derived from chronic HCV-patients and to detect some changes that might occur in their PB lymphocyte subsets (CD3+/4+/8+ and CDI6+/19+) by flowcytometery. Concerning caspase-8 specific activity, it was comparable in both groups, however, patients having DNA fragmentation
• (46.5%), showed statistically significant higher activity than those without detectable DNA fragmentation (p=0.03). All controls were negative for DNA fragmentation. In addition, a statistically significant positive correlation was found between absolute count of peripheral blood lymphocyte, CD3+, CDS+ and CD!9+ subpopulations of
.lymphocytes and plasma ALT level in patients with negativt• HCV-PCR. T:herefore, apoptosis is probably a major mechanism in pathogenesis of chronic HCV infection responsible for perpetuation of infection.
Other data
| Title | tA study of the process of apoptosis by measurement of the caspase-8 activity in peripheral blood T lymphocytes in chronic hepatitis C infected patients | Other Titles | دراسة عملية موت الخلايا المبرمج بقياس نشاط انزيم الكاسبيز 8 فى خلايا الدم (ت) الليمفاوية الطلافية فى المرضى المصابين بالالتهاب الكبدى الفيروسى (سى) المزمن | Authors | • Manal Said Fowzy | Issue Date | 2002 |
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