Biological Studies on the Radiosensitizing and Cytotoxic Properties of Some Newly Synthesised Chemical Compounds
Neimat Hanafy Ahmed;
Abstract
The goal of the present study is to high light the role of the newly synthesized chemical compound, namely zinc cysteine (Zn-cys), in cancer treatment. The role was studied either alone or in combination with gamma irradiation.
The present study is an in vitro and in vivo one.
7.1.0-In vitro study:
In vitro studies were carried out to demonstrate the effect of Zn-cys on Ehrlich tumor cells. It was demonstrated that increasing Zn-acetate concentration raise the percentage of non viable cells (N.V.C). However cysteine- HCI did not show any tumoricidal effect. The percentage of N.V.C. were proportionally increasing with Zn-cys concentration.
7.2.0-In Vivo Study:
In vivo studies were performed to illustrate the effect of Zn-cys on enhancing the theraputic effect of gamma irradiation.
The experimental models had been designed as follows, using female
Swiss albino mice and Ehrlich carcinoma (E.C) cell line.
Group I: Mice bearing tumor, inoculated with 2.5 X I 06 E.C. cells/ mice. Group 2: Mice bearing tumor and treated with 50mg/ kg Zn-cys, 24 hr
A.T.I (G2a) or 7 days A.T.I (G2b).
Group 3: Mice bearing tumor and exposed to 150, 300 and 600 R of gamma irradiation, 7 days A.T.I. to represent the subgroups G3a, G3b and G3c, respectively.
The present study is an in vitro and in vivo one.
7.1.0-In vitro study:
In vitro studies were carried out to demonstrate the effect of Zn-cys on Ehrlich tumor cells. It was demonstrated that increasing Zn-acetate concentration raise the percentage of non viable cells (N.V.C). However cysteine- HCI did not show any tumoricidal effect. The percentage of N.V.C. were proportionally increasing with Zn-cys concentration.
7.2.0-In Vivo Study:
In vivo studies were performed to illustrate the effect of Zn-cys on enhancing the theraputic effect of gamma irradiation.
The experimental models had been designed as follows, using female
Swiss albino mice and Ehrlich carcinoma (E.C) cell line.
Group I: Mice bearing tumor, inoculated with 2.5 X I 06 E.C. cells/ mice. Group 2: Mice bearing tumor and treated with 50mg/ kg Zn-cys, 24 hr
A.T.I (G2a) or 7 days A.T.I (G2b).
Group 3: Mice bearing tumor and exposed to 150, 300 and 600 R of gamma irradiation, 7 days A.T.I. to represent the subgroups G3a, G3b and G3c, respectively.
Other data
| Title | Biological Studies on the Radiosensitizing and Cytotoxic Properties of Some Newly Synthesised Chemical Compounds | Other Titles | دراسة بيولوجية على تأثير بعض المواد المخلقة صناعيا ً من حيث سميتها وتأثيرها الواقى للإشعاع | Authors | Neimat Hanafy Ahmed | Issue Date | 1999 |
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