CD14+ CD16+ MONOCYTES SUBPOPULATION LEVEL IN PATIENTS WITH ACUTE AND CHRONIC INFECTIONS UNDERGOING HEMODIALYSIS

Abstract

Infections are frequent complications in end-stage renal failure patients· undergoing hemodialysis (HD) and peripheral blood monocytes are important cells in host defense against infections. The majority of circulating monocytes express high levels of lipopolysaccharide receptor antigen CD14++and minor subpopulation coexpressing low level ofCD1/ together with CD1/ in peripheral blood of normal healthy persons. The aim of the work is to study the level of CD1 / CD1 / monocyte subpopulation in patients with chronic infection undergoing hemodialysis this study comprised 30 cases which was subjected to:

1. Detailed history with special emphasis on symptoms suggestive of infections.

2. Thorough clinical examination.

. 3. Renal function tests: Blood urea, serum creatinine, sodium, potassium, calcium, alkaline phosphatase.

4. Total leucocytic count and differential count with stress on monocytic count.

5. Determination of the level of CD1/ CD1/ monocyte subpopulation in the blood.

We studied these two monocytes subpopulations CD14++ and CD1/ CD1/ in HD patients (20 patients),10 controls.

In healthy controls (group I) consisting of 10 children 5 males and 5 females the CD14++ and CD14+ CD16+ number were (277.2±122.38 cells/J.tl), (24.4±14.03 cells/f.!!) and percentage (81.3%±14%), (6.8%±2.83%) respectively.

In HD patients without infection (group II) consisting of 10 patients (6 males and 4 females) the CD14++ and CD14+