Detection of Bacterial DNA in Pediatric Patients with Meningioencephalitis

Abstract

he present cross sectional study was designed to compare bacterial identification by detection of bacterial 16S rDNA as adiagnostic tool in diagnosis of children with bacterial meningitis attended Pediatric Emergency Department, Children's hospital, IMBABA FEVER hospitals, during the period between November 2015 to September 2016, compared to the diagnosis by bacterial isolation by culture. This study included 54 children presenting with fever, neck stiffness, convulsion, altered level of consciousness, all patients clinically diagnosed with bacterial meningitis, their ages ranged from 1month to 16 years,  54 patients were recruited according to inclusion criteria Clinically: All patients aging from 1 month -16 years with sudden onset of fever (>38.5°C rectal or 38.0°C axillary) and one of the following signs: neck stiffness, altered consciousness or other meningeals ign. Laboratory: Pleocytosis, predominantly of neutrophils, in CSF biochemical laboratory analysis.