EFFECT OF ADDITION OF SOME PLANT EXTRACTS TO ANTIBIOTICS ON PATHOGENIC STAPH.AUREUS

Abstract

Searches for substance with antimicrobial activity are frequent and medicinal plants have been considered interesting by some researches since they one frequently used in popular medicine as remedies for many infectious diseases. The aim of the study was to verify the antibacterial effect of aqueous, methanolic extracts and their essential oils of plants [clove (Syzygium aromaticum), mint (Mentha piperita), Thyme (Thymus vulgaris) , Garlic (Allium sativium), Sage (Salvia officinalis)] against 5 isolates of S .aureus isolated from pyogenic infections. The agar well diffusion method was the antimicrobial susceptibility performed test. S .aureus isolated from pleural fluid (P.F) out of five S .aureus (coagulase +ve, MSSA) exhibits resistant against all the concentration of Ciprofloxacin 0.5-20, Gentamycin 0.5- 5.0 µg/100µl and Amikacin 1 µg/100µl . The highest potent of phytoextracts either extracted by water or methanol was detected by clove comparing with mint , thyme , sage and garlic against all isolate, whereas garlic essential oil gave completely abolish of S .aureus P.F & T.B. Among 149 trials of combination between the lowest concentration of four antibiotics and different phytoextracts with different ratios against five pyogenic bacterial isolates .The combination between gentamycin and garlic essential oil with ratio 1: 3 represent high potent synergism against B.F, A.F and W.L (boils & abscess in face and wound in leg) isolates. Whereas, the combination between the same antibiotic and thyme essential oil with 1:3 gave high synergism against P.F and T.B isolates of S .aureus .The combination between garlic Eso + lowest concentration of Gentmycin (1:3) increment the synergism by 6.4, 2.5 and 6.0 fold against B.F , A.F and W.L isolates respectively comparing with standard concentration of gentamycin.