OPTIMISING OUTCOMES IN ASSISTED REPRODUCTION ( IUI, IVF, ICSI ) LABORATORY AND CLINICAL ASPECTS

Abstract

From this work it could be concluded that:

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• Regarding the best culture system and media for blastocyst culture, it could be concluded that our own laboratory made medium either in the routine or the transfer protocol and G1.2, G2.2 media can support equally blastocyst culture and viability.

• Wether the non significant better results obtained with the transfer system could reach a significant level when the number of embryos increases requires further investigations.

• Also, it could be concluded from our study that difference in Glucose uptake and Lactate production could be seen between embryos of different developmental stages.

• However, with the available routine methods used in the biochemistry it is not possible to differentiate between embryos of the same developmental stage but of varying qualities on the basis of their glucose uptake and lactate production.

• Thus choosing embryos for transfer on the basis of their nutrient uptake and lactate production could not be used at the moment as a part of the routine daily laboratory work.

• No significnat difference regarding glucose uptake and lactate production could be detected between.- day 5 embryos of IVF and ICSI.

• lUI Combined with active ovulation induction using C.C. is a good option for cases of cervical hostility.