BIO-CONTROL OF SOME PATHOGENS INFECTING DATE PALM USING VIRUSES
LOAY LEWAA EL-HAMD ABD-ALLAH;
Abstract
Date palm (Phoenix dactylifera L.) is one of the most important tropical and subtropical fruit tree in Egypt and in the Arabian region. Date palm belongs to genus phonix in the family Arecaceae that monocotyledon, dioecious plant. The Arabic terms of maturation stages of dates are Hababouk, kimri, khalal, rutab and tamar respectively.
In the present work, different studies on bacterial diseases that infected date-palm and their specific bacteriophages were carried out. The results can be summarized as follows:
Bacillus subtilis that considered pathogenic bacteria on date palm that caused systemic wilt and external malformation on date palm in tissue culture. Bacteria was obtained from central laboratory of date palm, Agricultural research center (ARC) Giza, Egypt
The other source of pathogenic bacteria was isolated from infected date palm exhibited dark pink and black spots on trunk, the flowers were colored with pink and in later stages flowers were colored black. Bacteria was isolated from date palm trunk that obtained from Agricultural research center (ARC) Giza, Egypt. The bacteria was isolated on NA medium and streaked on EMB and XLD media.
The pathogenicity test of B.subtilis and the isolated bacteria was carried out and same symptoms were appeared on plants.
The isolated bacterium was identified using light microscope and was found to be Gram negative, rod shape, single, non-spore forming. TEM showed that bacterium is short rod with length 1.8 micron and peritrichous flagella. Physiological method using bergey’s Manual referred to the isolated bacterium is Serratia marcescens. Molecular method using 16SrDNA sequencing showed that the isolated bacterium is Serratia marcescens.
In the present work, different studies on bacterial diseases that infected date-palm and their specific bacteriophages were carried out. The results can be summarized as follows:
Bacillus subtilis that considered pathogenic bacteria on date palm that caused systemic wilt and external malformation on date palm in tissue culture. Bacteria was obtained from central laboratory of date palm, Agricultural research center (ARC) Giza, Egypt
The other source of pathogenic bacteria was isolated from infected date palm exhibited dark pink and black spots on trunk, the flowers were colored with pink and in later stages flowers were colored black. Bacteria was isolated from date palm trunk that obtained from Agricultural research center (ARC) Giza, Egypt. The bacteria was isolated on NA medium and streaked on EMB and XLD media.
The pathogenicity test of B.subtilis and the isolated bacteria was carried out and same symptoms were appeared on plants.
The isolated bacterium was identified using light microscope and was found to be Gram negative, rod shape, single, non-spore forming. TEM showed that bacterium is short rod with length 1.8 micron and peritrichous flagella. Physiological method using bergey’s Manual referred to the isolated bacterium is Serratia marcescens. Molecular method using 16SrDNA sequencing showed that the isolated bacterium is Serratia marcescens.
Other data
| Title | BIO-CONTROL OF SOME PATHOGENS INFECTING DATE PALM USING VIRUSES | Authors | LOAY LEWAA EL-HAMD ABD-ALLAH | Issue Date | 2017 |
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