Biochemical and molecular studies on the mode of action of some isolated or synthetic new biologically active compounds

Abstract

In the present study, experiments were carried out to search for new natural antibiotics through isolation of various Actinomycete strains from different soil samples from Bayt al-Faqih area - Al Hudaydah governorate- west of Yemen and studying the antimicrobial effects of metabolites they produce. The isolate (S37) which was found to produce a wide spectrum antimicrobial agent was selected and identified. The cultural, physiological and biochemical characteristics of the isolate identified the strain as a member of the genus Streptomyces.

The parameters controlling the biosynthetic process of the antimicrobial agent including; different pH values, different incubation period, and different carbon and nitrogen sources, were fully investigated and the antibiotic was produced at the optimum condition. The antibiotic was extracted using chloroform: ethyl acetate (1:1). The purification of the antibiotic was performed using column chromatography technique. The purified antibiotic produced by isolate S37 was given the name Faqihmycin.