Detection of Epstein-Barr Virus in Non-Hodgkin's Lymphoma by ELISA and Polymerase Chain Reaction

Abstract

This study included 37 subjects from the NCJ,Cairo University.The cases studied were 12 cases of large cell lymphoma ,4 cases of inununoblastic lymphoma, 3 cases of Burkitt' s lymphoma , 4 cases were mixed small and large cell lymphoma ,4 cases of lymphoblastic lymphoma and 3 cases of monocytoid B-cell lymphoma. There are 7 subjects as controls.

The data reported in this study were:

1-Amplificd l'CR product of EBV-DNA detected by DNAenzyme immunoassay in circulating lymphocytes: In large cell and immunoblastic lymphoma( Group II) 56% were positive lor the EBV genome. Where the mean ± S.D was 0.424± 0.328 ,which was significant to the control and other lymphomas go ups . In other lymphomas (GroupIll) 46% were positive for the EBV-DNA ..Whcre mean± SD was 0.188 ± 0.084 which was significant to the control group.

2- Amplified PCR product of EBV-DNA detected by DNA enzyme immunoassay in lymph node biopsies: In large cell and inununoblastic lymphomas(Gruupll) EBV- DNA was in 44% of the cases. Where their mean ± S. D was 0.257 ± 0.2324 .This was statistically significant to control. In the other lymhpomas (Group Ill)