Clinical and Histopathological Evaluation of Regenerative Potential of Immature Permanent Teeth with Necrotic Pulp After Different Dentin Surface Treatments

Abstract

The aim of the present study was to assess the regenerative potential of young permanent immature nonvital teeth following different dentin surface treatments. The study included histopathologic, clinical and radiographic changes. The study consisted of two parts; animal and human parts. The animal part was performed for histopathologic and radiographic evaluation of the treatment outcomes over three months of follow-up. The total number of teeth (144) was divided into three equal groups according to the post-treatment evaluation period; group I, group II and group III for evaluation periods of two weeks, six weeks and three months respectively. Each group included 48 teeth and was further subdivided into 36 experimental teeth and 12 control teeth. The experimental samples were also subdivided into six equal subgroups according to the treatment protocol: subgroup (A) Blood Clot, subgroup (B) Blood Clot + Collagen, subgroup (C) Blood Clot + EDTA, subgroup (D) Blood Clot + EDTA + Collagen, subgroup (E) Blood Clot + MTAD, subgroup (F) Blood Clot + MTAD + Collagen and two control subgroups; subgroup (G) positive control Summary & Conclusion