Circulating endothelial cells in acute myeloid leukemia
AI shaymaa Ahmed Ibrahim;
Abstract
SummWJ'
SUMMARY
Many studies have demonstrated the presence of mature circulating endothelial cells (CECs) in the peripheral circulation (Beerepoot et at,
1004). However CECs are extremely rare events in normal peripheral blood, representing somewhere between 0.01% and 0.0001% of peripheral mononuclear cells (Khan et at, 1005). Circulating endothelial cells present in peripheral blood comprised of mature resting (rCEC) and activated (aCEC) circulating endothelial cell and endothelial progenitor cell (CEPC) (Wierzbowska eta/, 1005).
EPCs have properties similar to those of embryonic angioblasts (Beerepoot) et at, 1004) which can be defined as migratory endothelial cells with the capacity to circulate, proliferate and differentiate into mature endothelial cells (Khan el a/, 1005) but which have not yet acquired characteristic mature endothelial markers and have not yet formed a lumen (Zampetaki et at, 1008).
A key step for tumor growth and metastasis is the establishment of new blood vasculature. Angiogenesis is the sprouting of new blood vessels from already-established vessels; this is in contrast to vasculogenesis, which refers to the de novo fonnation of blood vessels from endothelial progenitors (Gao and Mittal/, 1009).
Recent data suggest that endothelial cells may enhance the survival and proliferation of leukemic blasts and mediate chemotherapy resistance in acute myeloid leukemia (AML). In peripheral blood of AML patients there's an increase in the amount of CECs which correlate with disease status and response to therapy supporting the idea that CEC could represent a marker of angiogenesis (Wierzbmvska el al, 1005).
The aim of this work was to study aCEC and EPC levels in patients with AML using flow cytometric analysis (lyse no wash technique) and compare their levels with healthy subjects to prove their role in tumorigenesis and correlate these findings with clinical and hematological data of patients and their response to treatment at the 28'11 day of treatment.
This study was conducted on fifty de novo AML patients who subjected to full history taking, proper clinical examination and routine chemical
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SUMMARY
Many studies have demonstrated the presence of mature circulating endothelial cells (CECs) in the peripheral circulation (Beerepoot et at,
1004). However CECs are extremely rare events in normal peripheral blood, representing somewhere between 0.01% and 0.0001% of peripheral mononuclear cells (Khan et at, 1005). Circulating endothelial cells present in peripheral blood comprised of mature resting (rCEC) and activated (aCEC) circulating endothelial cell and endothelial progenitor cell (CEPC) (Wierzbowska eta/, 1005).
EPCs have properties similar to those of embryonic angioblasts (Beerepoot) et at, 1004) which can be defined as migratory endothelial cells with the capacity to circulate, proliferate and differentiate into mature endothelial cells (Khan el a/, 1005) but which have not yet acquired characteristic mature endothelial markers and have not yet formed a lumen (Zampetaki et at, 1008).
A key step for tumor growth and metastasis is the establishment of new blood vasculature. Angiogenesis is the sprouting of new blood vessels from already-established vessels; this is in contrast to vasculogenesis, which refers to the de novo fonnation of blood vessels from endothelial progenitors (Gao and Mittal/, 1009).
Recent data suggest that endothelial cells may enhance the survival and proliferation of leukemic blasts and mediate chemotherapy resistance in acute myeloid leukemia (AML). In peripheral blood of AML patients there's an increase in the amount of CECs which correlate with disease status and response to therapy supporting the idea that CEC could represent a marker of angiogenesis (Wierzbmvska el al, 1005).
The aim of this work was to study aCEC and EPC levels in patients with AML using flow cytometric analysis (lyse no wash technique) and compare their levels with healthy subjects to prove their role in tumorigenesis and correlate these findings with clinical and hematological data of patients and their response to treatment at the 28'11 day of treatment.
This study was conducted on fifty de novo AML patients who subjected to full history taking, proper clinical examination and routine chemical
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Other data
| Title | Circulating endothelial cells in acute myeloid leukemia | Other Titles | دراسة نسبة خلايا الغشاء المبطن للاوعيه الدمويه السابحه فى الدم فى مرضى سرطان خلايا الدم البيضاء النخاعى الحاد | Authors | AI shaymaa Ahmed Ibrahim | Issue Date | 2010 |
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