Molecular Study Of Apolipoprotein B Gene In Ischemic Heart Diseases And Its Association With Hypercholesterolemia

Abstract

Cardiovascular disease is the most important cause of death in the world today, Ischemic heart disease includes a wide spectrum of conditions, ranging from silent ischemia and exertion induce angina, through unstable angina to acute myocardial infarction (MI). Coronary artery disease has become a major cause of morbidity and mortality in Egypt Atherosclerosis is a systemic disease with independent rates of occurrence in coronary arteries. This study aims to evaluate the role of apolipoprotein (apo)B gene mutation of all potentially atherogenic lipoproteins, as predictors of Ischemic Heart Diseases (IHD) and its association with other biochemical changes. The results presented revealed the following data: Fasting and postprandial blood glucose was highly significant increase (p<0.001) in groups II and III when compared with control group. Also there are significant increase of blood urea level (P<O.OS) in group II, also; highly significant increase of both blood urea in group III compared to control group. Serum creatinine, urea and BUN were not correlated to the hyperglycemia in all studied groups. The major abnormalities were the highly significant changes (p <0.001, 0.01) of triacylglycerol, VLDL-c, LDL-c and total cholesterol in all studied groups compared to control group, while, HLD-c was significantly decrease all studied groups compared to control group. There was significant increase of FFA in group one and non significant changes in group II and III compared to control group. Also, no significant changes of phospholipids in all studied groups compared to control group. The percentage of homozygous in groups I, II and III apo B gene was expressed by (73%), (40%}, and (86.7%) respectively .The percentage of heterozygous in groups I, II and III apo B gene was expresedby(27%},(60%)and(13.3%) respectively. In conclusion: Apo B expression could be a good criterion for characterizing the patients suffering from ischemic heart disease. Farther work is strongly recommended for the possibility of using the previous biochemical parameters for diagnosis, prognosis and therapy for ischemic heart disease.