DESIGN AND CONSTRUCTION OF ULTRASOUND APPARATUS FOR BIOLOGICAL CELLS MANIPULATION AND SEPARATION

Abstract

The trapping and manipulating of target cells and suspended particles from a medium is of great importance to cell biology, drug delivery, and related fields in biomedicine. Furthermore, the ability to trap and separate cells, which have tremendous medical applications, is of profound interest to the biomedical community. In the, present work, design and con.struct of a home-made trappiq.g device to

manipulate and separate cells were conducted using ultrasonic standing waves. The effect of various experimental conditions on trapping efficiency was conducted. The performance two ultrasonic trapping chamber (cylindrical and cuboidal ) at 0.8 MHz, different intensities ranged from 0.5-3 W/cm2 continuous

wave ultrasound were investigated. Three different cell types (R.B.Cs, yeast and, E.coli) were used to investigate the cell size effect on trapping behavior. The effect of media concentration on trapping time and velocity of the cell was investigated. Monitoring of the cells behavior during ultrasonic manipulation by using three

simple techniques (light microscopy, dielectric measurement and optical absorption technique) was studied. • Light microscopy inspection reflects an instantaneous movement of the

cells during trapping.

• Dielectric measurement gives information about electrical properties of the cells. • Optical absorption measured the transmitted light passes through the

trapping chamber.

The experimental results showed that:

• Ultrasonic standing wave cell trapping and separation technique provides accurate, low power consumption with minimal damage to cells in addition to high throughput.