Biophysical study of some physical properties of mammalian blood THESIS
Naglaa Mostafa Mohamed Ismail;
Abstract
The present work, aimed to study the effect of some chemicals compounds (ascorbic, acid, ferric sulphate, copper sulphate, zinc
• sulphate,and manganese sulphate) on the biophysical properties of erythrocytes suspended in saline solution with concentration of
4.7x107 cell/cm3 .
The effect was studied by recording the change in the absorbance at
577 run of that suspension due to the addition of that compounds with different concentrations. Ascorbic acid (vitamin c) cause hemolysis of erythrocytes suspension and this process was accelerated by in creasing its concentration.
The addition of metals (Zn, Mn &Cu) in the form ofsulphates on erythrocytes suspension gave no hemolysis effect, while ferric sulphate can induce hemolysis and this process was accelerated by increasing the concentration of ferric sulphate.Showed that the relative hemolysis time decreases with increasing the concentration of metals in the presence of ascorbic and while relative hemolysis rate increases with increasing the concentration .The effect of
manganese of concentration<1o•3M on erythrocytes resulted in an
acceleration of hemolysis relative to control values while manganese of concentration> 1o•3M on erythrocytes resulted in
deceleration of hemolysis relative to control values.
In general the increase in Mn concentration decelerate the process of hemolysis in the presence of ascorbic acid.
The above observation obtained also in the conductivity
measurements.
The permittivity of blood, red blood cell, Hb,red blood cell after hemolysis are recorded and showed that The permittivity of red blood is the highest one and that of red blood after hemolysis is the lowest one.
The measurements of conductivity showed that, red blood cell has
the highest conductivity and while the conductivity of red blood cells after hemolysis by different metals approximately the same. Key words: erythrocytes,hemolysis,ascorbic acid, metals
,permittivity. Electrical conductivity
• sulphate,and manganese sulphate) on the biophysical properties of erythrocytes suspended in saline solution with concentration of
4.7x107 cell/cm3 .
The effect was studied by recording the change in the absorbance at
577 run of that suspension due to the addition of that compounds with different concentrations. Ascorbic acid (vitamin c) cause hemolysis of erythrocytes suspension and this process was accelerated by in creasing its concentration.
The addition of metals (Zn, Mn &Cu) in the form ofsulphates on erythrocytes suspension gave no hemolysis effect, while ferric sulphate can induce hemolysis and this process was accelerated by increasing the concentration of ferric sulphate.Showed that the relative hemolysis time decreases with increasing the concentration of metals in the presence of ascorbic and while relative hemolysis rate increases with increasing the concentration .The effect of
manganese of concentration<1o•3M on erythrocytes resulted in an
acceleration of hemolysis relative to control values while manganese of concentration> 1o•3M on erythrocytes resulted in
deceleration of hemolysis relative to control values.
In general the increase in Mn concentration decelerate the process of hemolysis in the presence of ascorbic acid.
The above observation obtained also in the conductivity
measurements.
The permittivity of blood, red blood cell, Hb,red blood cell after hemolysis are recorded and showed that The permittivity of red blood is the highest one and that of red blood after hemolysis is the lowest one.
The measurements of conductivity showed that, red blood cell has
the highest conductivity and while the conductivity of red blood cells after hemolysis by different metals approximately the same. Key words: erythrocytes,hemolysis,ascorbic acid, metals
,permittivity. Electrical conductivity
Other data
| Title | Biophysical study of some physical properties of mammalian blood THESIS | Other Titles | دراسة بيوفيزيائية لبعض الخواص الفيزيائية لدم الثدييات | Authors | Naglaa Mostafa Mohamed Ismail | Issue Date | 2005 |
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