Molecular Characterization of Mycoplasma gallisepticum in Chicken.

Abstract

Abstract: Mycoplasma gallisepticum (MG) is an important avian pathogen causes significant economic losses within the poultry industry. One of the options of the controlling MG infection is the isolation and identification of MG. Two hundreds and fifteen samples collected from the lungs, tracheal bifurcation, tracheal swabs and frontal sinus aspirates and 80 blood samples were collected from broiler and layer flocks in Damietta governorate. Organ and tracheal swab samples were cultivated on Frey’s media giving 142 samples positive as (Order. Mycoplasmatales) as 66%. The 142 samples was used for digitonin sensitivity test and the results were 98 samples as digitonin positive (F. Mycoplasmataceae) 69% and 44 samples was digitonin negative (F. Acholeplasmataceae) 31%.One hundred and ninety six samples were characterized by PCR by using MG primer. The samples had amplified fragments at 300 bp against MG. Four samples were used for gene targeted sequencing.