Gene expression profiling in hepatic fibrosis patients with chronic hepatitis c virus infection using expression array of fibrogenesis

Abstract

fibrosis related genes in peripheral blood mononuclear cells (PBMCs). The RNA from 30 treatment-naïve chronic HCV patients (17 F0-F2 and 13 F3-F4) and 6 healthy subjects were enrolled. Validation of the results were performed by custom real time PCR in 75 treatment-naïve chronic HCV patients (39 F0-F2 and 36 F3-F4) and 10 controls. From the first approach data (PCR array data), selected eleven genes were based on their fold change, physiological pathway and p value. In the second approach, custom real time PCR confirmed that expression of transforming growth factor β (TGF β1) gene at the early stage of fibrosis is highly regulated compared to control (fold differential regulation =2.555033, p= 0.035), whereas the TG- interacting factor (TGIF1) gene which is the inhibitory of TGF β1 is down regulated (fold differential regulation =-1.2, p= 0.041). At the late stage of fibros