Bacterial Reduction after using different Irrigation protocols in Healthy and Diabetic patients with Apical Periodontitis

Abstract

The purpose of this investigation was to estimate bacterial reduction after using different irrigation protocols in healthy and diabetic patients with apical periodontitis. Forty patients(20 healthy and other 20 diabetic) participated in this study. They were selected from those admitting for endodontic treatment at endodontic department, Faculty of dentistry, Ain shams university as well as at Diabetes institute dental clinic. Group A Include twenty healthy patients with no history of any systemic disease with necroric pulp with chronic apical periodontitis. While Group B Include twenty controlled diabetic type two patients with necroric pulp with chronic apical periodontitis. Preoperative and post operative samples were collected from each case for estimating the bacterial reduction for Fusobacterium Nucleatum, Peptostreptococcus Micros, Entrococcus Fecalis, before and after treatment by quantitative real time polymerase chain reaction. Root canal preparation was performed with the one shape single file system and irrigation was performed either with conventional side vented syringe or Endo vac device. Statistical analysis of results showed that; for Fusobacterium Nucleatum; Regarding the effect of irrigation techniques , there was no statistically significant difference in Fusobacterium Nucleatum reduction after irrigation with Conventional Syringe irrigation or Endo vac regardless of the systemic health of the patients (p>0.05). Whereas; Regarding the effect of systemic health of the patients, there was no statistically significant difference in Fusobacterium Nucleatum count reduction between healthy and diabetic patients, regardless of the method of irrigation used (p>0.05). For Peptostreptococcus Micros; Statistical analysis of these results showed that; Regarding the effect of irrigation techniques , There was no statistically significant difference in Peptostreptococcus Micros reduction after irrigation with either Conventional Syringe irrigation or Endo vac in the healthy group (p= 0.68ns). while there was statistically significant difference in the diabetic group(p= 0.05ns). While; Regarding the effect of systemic health of the patients, There was no statistically significant difference in Peptostreptococcus Micros reduction between healthy and diabetic patients regardless of the method of irrigation used (p>0.05). For Enterococcus Fecalis: Statistical analysis of these results showed that; Regarding the effect of irrigation techniques, There was no statistically significant difference in Enterococcus Fecalis count reduction after Conventional Syringe irrigaion or Endo vac , regardless of the systemic health of the patients (p>0.05). while Regarding the effect of systemic health of the patients, There was no statistically significant difference in bacterial reduction between healthy and diabetic patients, regardless of the method of irrigation used(P>0.05). Furthermore there was no statistical significant difference for the overall bacterial count reduction regardless of its type, systemic health of the patient or irrigation method used.