Isolation and Identification of the medically important bacteria associated with hospital insects

Abstract

Insects were surveyed in two hospitals (ASUSH, urban) and (BUH, rural) with different hygienic levels and their adjacent residential areas (ASUSHC and BUHC for the two hospitals, respectively) to isolate and identify bacteria associated with such insects. A total of 5257 adults were collected of which Dipterous flies were the abundant ( 72 % insect) and Muscadomesticawas the most abundant species (67 % insect) which was present in all areas where it was more common / predominant species (22 %-91% insect), also M. domesticahad the maximum bacterial carriage (10spp., 63%). Moreover, higher densities of M. domesticawerein BUH and BUHC than in ASUSH or ASUSHC. The heavily infested area was ASUSHC(55% species) followed by ASUSH, BUHC and BUH however, the total number of the collected insects was higher in BUHthan in ASUSH. In all areas, M.domesticawas more common during summer/ autumn and spring than in the winter.Periplanetaamericana collected only during autumn in ASUSHCand was more common in autumn in BUHC while Blatellagermanica collected only during summer in ASUSHC and was more common in autumn in BUH. Seventeen bacteria spp. were isolated and identified from the external surface and internal organs of the collected insectsof which,AcinetobacterandKlebsiella were the most common (5 insect spp., 31% each) followed by Anthracoid, Enterobacter, E.coli, MRS-ve, Bacillus, Stenotrophomonas, MRSA, Pseudomonas, Citrobacter, Raoultella, Salmonella,Enterococci, Staph.Coag –ve , Non haemolyticstrept and Diphtheroid.The present study emphasis the importance of insects mainly housefly and cockroaches as potential vectors of pathogenic bacteria in hospital environments specially rural ones. So that fly and cockroach control strategies as well as hygiene promotion programs should be applied to avoid or eliminate the health risks to the people in residential areas and transmission of nosocomial infections to patients and workers in the hospitals.