ANTIVIRAL SUBSTANCES INDUCED IN PLANTS AS A RESULT OF VIRUS INFECTION
SABRY YOUNIS MOHAMED MAHMOUD;
Abstract
Bean yellow mosaic virus (BYMV) was isolated depending on symptoms, ELISA and electron microscope from different governorates during season 1996. Tomato mosaic virus (ToMV) isolate was identified by indicator hosts and electron microscopy. It was found that C. amarantico/or is the suitable hypersensitive host to both viruses, based on acquired resistance and SDS-PAGE.
In leaves of Chenopodium amarantico/or locally infected by Tomato mosaic virus (ToMV) or Bean Yellow Mosaic Virus (BYMV), antiviral substance (AVS) was formed after 5 and 6 days from inoculation respectively in inoculated and uninoculated leaves as inhibitor of virus .biosynthesis. Crude AVS was extracted by hydrated calcium phosphate and it was purified by DEAE - column chromatography. AVS was acquired systemic resistance against a virus-challenge inoculation. AVS consists of protein and carbohydrate (phosphorylated - glucoprotein) with a molecular weight of
19,400 and 21,500 Kda.
AVS lost its antiviral activity when treated with alkaline phosphate and a-glucosidase, while it does not when treated with l ipase as well as it is inhibited by actinomycin D. This lead to thjnk that its fonnation depends on the same mecharusm which is responsible for the synthesis of cellular RNA, DNA dependent.
By non-denaturing PAGE, AVS is separated into one antiviral active band in both ToMV and BYMV. SDS-PAGE in denaturing conditions revealed at least two new bands with respect to compared extracts of healthy plants.
The AVS-product is production of non-specific antiviral substances in a general, defence response in plants to viral infections, which could be utilized for practical purposes, such as the use of AVS treatments under green-house conditions and in open field, or the production of transgenic plants producing AVS in the future.
In leaves of Chenopodium amarantico/or locally infected by Tomato mosaic virus (ToMV) or Bean Yellow Mosaic Virus (BYMV), antiviral substance (AVS) was formed after 5 and 6 days from inoculation respectively in inoculated and uninoculated leaves as inhibitor of virus .biosynthesis. Crude AVS was extracted by hydrated calcium phosphate and it was purified by DEAE - column chromatography. AVS was acquired systemic resistance against a virus-challenge inoculation. AVS consists of protein and carbohydrate (phosphorylated - glucoprotein) with a molecular weight of
19,400 and 21,500 Kda.
AVS lost its antiviral activity when treated with alkaline phosphate and a-glucosidase, while it does not when treated with l ipase as well as it is inhibited by actinomycin D. This lead to thjnk that its fonnation depends on the same mecharusm which is responsible for the synthesis of cellular RNA, DNA dependent.
By non-denaturing PAGE, AVS is separated into one antiviral active band in both ToMV and BYMV. SDS-PAGE in denaturing conditions revealed at least two new bands with respect to compared extracts of healthy plants.
The AVS-product is production of non-specific antiviral substances in a general, defence response in plants to viral infections, which could be utilized for practical purposes, such as the use of AVS treatments under green-house conditions and in open field, or the production of transgenic plants producing AVS in the future.
Other data
| Title | ANTIVIRAL SUBSTANCES INDUCED IN PLANTS AS A RESULT OF VIRUS INFECTION | Other Titles | مضادات الفيروس المستحثة في النباتات كنتيجة للاصابة الفيروسية | Authors | SABRY YOUNIS MOHAMED MAHMOUD | Keywords | BYMV, ToMV, Induced resistance, ELISA, Hypersensitivity, Antiviral substances (AVSs), DEAE-Cellulose column chromatography, Gel filtration column chromatography and SDS PAGE. | Issue Date | 2000 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| SABRY YOUNIS MOHAMED MAHMOUD.pdf | 2.45 MB | Adobe PDF | View/Open |
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