Regenerative potentials of post natal stem cells in non vital immature teeth (An Animal study)

Abstract

This study was an animal study which was performed for radiographic and histopathologic evaluation of the treatment outcomes over 3 months of follow up. The total number of teeth (48), was divided into 2 equal groups according to the post treatment evaluation period; group I and group II for: Evaluation periods of one month and three months respectively. Each group included 24 teeth and was further subdivided into 16 experimental teeth and 8 control teeth. The experimental samples were also subdivided into 2 subgroups according to the treatment protocol: Subgroup (A): Revascularization (8 teeth), Subgroup (B): Scaffold impregnated with BMP-2(Infuse bone graft) and DPSCs (8 teeth) and two control subgroups; subgroup (C) Positive control and subgroup (D) Negative control. Each individual root was taken as the unit of measure. Each subgroup was both radiographically and histopathologically evaluated. Induction of infection was done followed by canal disinfection using the triple antibiotic paste. Treatment protocols were performed according to the previous classification and samples were left for follow up according to the period for each group.