Assessment of photodynamic treatment versus medical treatment of Helicobacter pylori using PCR

Abstract

Helicobacter pylori is one of the most common causes of chronic bacterial infections in humans and is important in the pathogenesis of gastrointestinal disease, such as duodenal ulcer, gastric ulcer, Gastric adenocarcinoma, and lymphoma. Gastric adenocarcinoma remains one of the leading causes of cancer death in the world. The objective of this study was to assess the effect of photodynamic treatment and medication treatment of Helicobacter pylori using RAPD-PCR. H.pylori was isolated from gastric biopsy specimens during endoscopy, by culturing and isolating the bacterium under microaerobic conditions. H.pylori was diagnosed by visualization of the bacterium by light microscopy and by the characterizing enzymes (urease, catalase and oxidase) it produces. The lethal photosensitization effect was determined by mixing suspensions of H.pylori with Toluidine blue 0 (TBO) and plating_ out on blood agar before irradiation with Helium neon (He-Ne) 632.8 nm. The susceptibility of Helicobacter pylori isolates to metronidazole and (}... ,Azithromycin were examined by E-test, where they were sensitive to o fizithromycin and resistant to Metronidazole. Nine J3..Kf>D primers were

used to screen genetic polymorphism in DNA of different H.pylori groups. Six of them produced RAPD products while three failed to generate any product. Two bands (No. 12, 416 bp & no.15, 268 bp) may represent genetic marker for H.pylori species or higher taxonomic position on family or order levels but further techniques should be applied to verify the identity of these markers. The resulting data showed