The influence of a selective hypoglycemic and immunosuppressive drugs on dog's pulp tissue healing

Abstract

The aim of the present study was designed to evaluate the influence of hypoglycemic agent (insulin) and immunosuppressant agent (azathioprine) on pulp tissue healing after pulp capping using MTA, BioAggregates and calcium hydroxide. Eight healthy dogs were used for the study and classified into four groups: [Immunosuppressed (G1); Normoglycemic (G2); Hyperglycemic (G3); and Control (G4)]. A total of 120 teeth were used (15 teeth in each dog). Four dogs sacrificed after one month of operative procedure and the other ones were after two months. The groups were treated preoperatively as follows: G1 treated by azathioprine for two months. In G2 and G3, diabetes was induced two weeks preoperatively by I.V. injection of alloxan; then insulin was given to control blood glucose level (BGL). The amount of the injected insulin was adjusted to be as close as normal in G2 (Normoglycemic group) and to high level in G3 (hyperglycemic group). G4 (control group) had no treatment before and\or after operative procedure. Class V buccal cavities were prepared in the teeth approximately 1 mm coronal to the gingival margin with no. 2 inverted cone carbide at high speed under copious normal saline irrigation. Sterile sharp probe was used mechanically to expose the pulp. After controlling the bleeding, the tested materials were applied as indicated by the manufacturers. After the end of each experimental period, dogs were euthanized using 20 ml of 5% thiopental sodium solution rapidly injected through the Summary 133