Some Pharmacological Effects of Cynara Scolymus (Artichoke) Leaves Extract
Khalid Gaith Hashim Abd-El-rhman;
Abstract
The present study was performed to qualitatively determine the bioactive chemical constituents of Cynara scolymus (Artichoke) leaves extract (CSLE) and to estimate its oral acute toxicity (LD50). The antimicrobial activity of CSLE against some microorganisms was carried out in vitro. The hepatoprotective effect of this extract against acute hepatotoxicity induced by CCL4 in rats was performed. The effects on serum liver enzymes, total cholesterol, triglycerides, reduced glutathione, and malondialdehyde and blood urea nitrogen were carried out. Hepatic tissue antioxidant enzymes activity was determined and histopathology of liver was carried out. Some other pharmacological activities including antidiarrheal, antioxidant, antinociceptive (analgesic) and anti-inflammatory effects of CSLE were evaluated.
Antibacterial and antifungal activities of CSLE against some Gram positive, some Gram negative bacteria and some fungi were performed using Microtiter plate and the minimum inhibitory concentration (MIC) method.
Phytochemical screening of CSLE was performed using tests for the presence of alkaloids, saponins, glycosides, tannins, resins, sterol and/or triterpenes and flavonoids. The chemical tests were carried out using standard procedures.
The acute oral toxicity (LD50) of Cynara scolymus (Artichoke) leaves extract in mice was calculated mathematically according to Kerber method.
The hepatoprotective effect of this extract against acute hepatotoxicity induced by CCL4 in rats was performed. The experiment was carried out on 35 adult Sprague Dawley rats randomly distributed into 5 groups, of 7 rats each. Group (1) was kept (normal) negative control and the remaining 4 groups were subcutaneously injected by 2 ml/kg/rat of carbon tetrachloride CCL4 during the last week of experiment to induce acute hepatotoxicity. Group (2) was kept as a positive control (model) group. Groups (3), (4) and (5) were orally pretreated with the CSLE daily at doses of 200, 400 and 800 mg/kg b.wt, respectively for 6 weeks.
Antibacterial and antifungal activities of CSLE against some Gram positive, some Gram negative bacteria and some fungi were performed using Microtiter plate and the minimum inhibitory concentration (MIC) method.
Phytochemical screening of CSLE was performed using tests for the presence of alkaloids, saponins, glycosides, tannins, resins, sterol and/or triterpenes and flavonoids. The chemical tests were carried out using standard procedures.
The acute oral toxicity (LD50) of Cynara scolymus (Artichoke) leaves extract in mice was calculated mathematically according to Kerber method.
The hepatoprotective effect of this extract against acute hepatotoxicity induced by CCL4 in rats was performed. The experiment was carried out on 35 adult Sprague Dawley rats randomly distributed into 5 groups, of 7 rats each. Group (1) was kept (normal) negative control and the remaining 4 groups were subcutaneously injected by 2 ml/kg/rat of carbon tetrachloride CCL4 during the last week of experiment to induce acute hepatotoxicity. Group (2) was kept as a positive control (model) group. Groups (3), (4) and (5) were orally pretreated with the CSLE daily at doses of 200, 400 and 800 mg/kg b.wt, respectively for 6 weeks.
Other data
| Title | Some Pharmacological Effects of Cynara Scolymus (Artichoke) Leaves Extract | Other Titles | بعض التأثيرات الدوائية لخلاصة أوراق الخرشوف | Authors | Khalid Gaith Hashim Abd-El-rhman | Issue Date | 2017 |
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