Biochemical Studies For Evaluation and Molecular Characterization of a New Polymerase Chain Reaction-Enzyme-Linked Immunosorbent Assay (PCR-ELISA) for Detection of Wuchereria bancrofti DNA

Abstract

0 This work• has been carried out to evaluate the performance, sensitivity and practical utility of the newly developed polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) in detecting Wuchereria bancrofti DNA in the mosquito vector in a low endemic village as a model for after treatment situation. The described assay that combines the conventional PCR with the use of an internal contra and a very sensitive ELISA detection of PCR products can be successfully employed to screen large numbers of mosquitoes and calculate the infection rate of mosquitoes in low endemic areas. It was successfully employed to estimate the relative amount of W bancrofti DNA present in wild caught mosquitoes providing semi-quantitative data. The method provides accurate description of the level of inhibition in mosquito pools and thus help preventing false negative results.