Evaluation of the column agglutination . technology for ABO grouping and D typing
Nermine Mohamed Abd Elbadi Alsheikh;
Abstract
Since the early 1990s, tube agglutination has been used as a traditional method for ABO grouping and D typing.
Column agglutination technology has bee!l recently developed as an alternative method to overcome the inherent variations in pipetting reactants and inconsistency in grading the strength of the reaction.
The two techniques were compared for ABO grouping and D typing as well as for the detection of weak D phenotypes. 200 blood samples collected from blood donors at the blood donation center of The Egyptian Organization For Biological Products & Vaccines (VACSERA) were examined for ABO grouping and D typing by both column and tube agglutination assays in VacSera and the NBTC. Both techniques performed similarly as regards to ABO grouping and D typing.
Additional 30 D-negative blood samples collected from blood donors were tested for the detection of weak D phenotypes by both techniques. Again there was a similarity in the reaction patterns between both techniques.
We concluded that the column agglutination technology is as sensitive as the tube agglutination in ABO grouping and Du testing and more sensitive than tube agglutination in D typing. Being easier, standardized, and stable and overcoming the subjectivity of the tube agglutination, the column agglutination can be favorably used as an alternative to tube agglutination.
Column agglutination technology has bee!l recently developed as an alternative method to overcome the inherent variations in pipetting reactants and inconsistency in grading the strength of the reaction.
The two techniques were compared for ABO grouping and D typing as well as for the detection of weak D phenotypes. 200 blood samples collected from blood donors at the blood donation center of The Egyptian Organization For Biological Products & Vaccines (VACSERA) were examined for ABO grouping and D typing by both column and tube agglutination assays in VacSera and the NBTC. Both techniques performed similarly as regards to ABO grouping and D typing.
Additional 30 D-negative blood samples collected from blood donors were tested for the detection of weak D phenotypes by both techniques. Again there was a similarity in the reaction patterns between both techniques.
We concluded that the column agglutination technology is as sensitive as the tube agglutination in ABO grouping and Du testing and more sensitive than tube agglutination in D typing. Being easier, standardized, and stable and overcoming the subjectivity of the tube agglutination, the column agglutination can be favorably used as an alternative to tube agglutination.
Other data
| Title | Evaluation of the column agglutination . technology for ABO grouping and D typing | Other Titles | تقييم طريقة التلازن العمودي في تحديد فصائل الدم وتصنيف معامل ريسوس | Authors | Nermine Mohamed Abd Elbadi Alsheikh | Issue Date | 2001 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| Nermine Mohamed Abd Elbadi Alsheikh.pdf | 1.4 MB | Adobe PDF | View/Open |
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