Comparative study between oxygen therapy and rutin as an antioxidant in hyperlipidemic rats

Abstract

Obesity is a medical condition in which excess body fat has accumulated to the extent that it may have an adverseeffect on health, leading to reduced life expectancy and/or increased health problems. Obesity is often multifactorial, based on both genetic and behavioral factors. Overweight is a significant contributor to health problems. It increases the risk of developing a number of diseasesincluding Type 2 (adult-onset) diabetes, hypertension and myocardial infarction. The simple cause is ingestion of more calories than are required for energy, the excess being stored in the body asfat. Inactivity and insufficient exercise can be contributing factors; the less active the person, the fewer calories areneeded to maintain normal body weight. Overeating may result from unhealthful patterns of eating established bythe family and cultural environment, perhaps exacerbated by psychological distress, an emotional dependence onfood. Rutin is the most important flavonoid in herbal foods and exhibit health beneficial properties for humans, such as XOD inhibition, anti-hyperuricemia, anti-inflammation and anti hyperlipidemia. Previous studies confirmed that rutin lowered leptin, total cholesterol (TC), very low-density lipoprotein (VLDL), total triglyceride (TG) in HFD rats. So, the present investigation aimed to investigate the effects of path- physiological effect of HFD induced obesity in male albino rats and, the therapeutic effects of administration of oxygen therapy and rutin with antioxidant properties in ameliorating lipid profile is estimated. To attain this goal, a total number of 100adult male albino rats having an average weight of 150-170 g were used. The animals were divided into two main groups consequently divided into six subgroups. The first group representing non-obesity control group rats subdivide into: Normal control, control rutin orally injected with 50 mg/kg b. wt.; and control ozonized water orally injected with 0.5ml /100g.b. wt. Group two representing obesity group rats were fed HFD.It was then subdivided into three subgroups: Recovery group allowed a 60-day period of cessation of HFD feeding; obese rutin orally injected with 50 mg/kg b. wt.and obese ozonized water subgroup orally injected with 0.5 ml/100g b. wt. Five rats from each subgroup were randomly selected and dissected at 0, 30, 45 and 60 days experimental duration. At the end of experimental period, results obtained were as follows: - Histological studies Histological examination of liver tissue after HFD feeding showed histological changes illustrated by necrotic cells; Kupffer cells proliferation, infiltrative inflammatory monocyteshemorrhagicchange, fatty degeneration, fat vacuoles, cytoplasmic vacuolation of most of hepatocytes and loss of hepatic architecture. Liver sections of rats from recovery obesity subgroup treated with rutin at dose 50 mg/kg b.wt evidenced limited fatty degenerative change with dilaled sinusoid and necrotic hepatocytes On the other hand; many hepatocytes appeared more or less with present of amyelid degenerative after ozonized water administration at a dose 0.5 ml/kg b.wt. Histochemical staining of glycogen and protein contents revealed a time dependent increase in all parameters.