GENOTOXIC EFFECT OF METHOTREXATE ON BONE MARROW CHROMOSOMES AND DNA OF MALE ALBINO MICE Mus musculus
Sally Ramadan Gabr Eid El-Ashry;
Abstract
Methotrexate (MTX) is an antineoplastic, antipsoriatic and antirheumatic agent belongs to the group of antimetabolites as it inhibits folic acid metabolism. The present work is mainly concerned with the study of the genotoxic effect of it on bone marrow chromosomes and DNA of male albino mice Mus musculus.
Sixty CD-1 male mice of nearly the same age (16-18 weeks old) were individually weighed 24 ± 2 g and randomly divided into four groups, one control group and three treated groups with different doses of methotrexate. Each group consisted of fifteen mice. The control group was injected intraperitonealy with 1ml/kg b.wt. distilled water, while treated group (1) was intraperitoneally injected with 2.5 mg / kg b. wt., single dose at the first day of the experiment and sacrificed by cervical dislocation after 24, 48 and 72 hour of treatment, treated group (2) was intraperitoneally injected with methotrexate 5 mg / kg b.wt., single dose at the first day of the experiment and sacrificed by cervical dislocation after 24, 48 and 72 hour of treatment and treated group (3) was intraperitoneally injected with methotrexate 10 mg / kg b.wt., single dose at the first day of experiment and sacrificed by cervical dislocation after 24, 48 and 72 hour of treatment.
The present study showed that the normal mouse chromosomes are 40 telocentric chromosomes. They are divided into five distinct groups; each contains the chromosomes of nearly equal lengths.
The current results showed that the tested doses of methotrexate induced structural and numerical chromosomal aberrations in male albino mice bone marrow cells which were highly significant increased (P< 0.001) by dose and time. Structural aberrations were chromosomal and chromatid gaps, fragments, centromeric attenuation, deletion, centric fusion, ring formation, end to end association and beaded chromosomes. Also, methotrexate treatment caused numerical aberration in the form of polyploidy.
Sixty CD-1 male mice of nearly the same age (16-18 weeks old) were individually weighed 24 ± 2 g and randomly divided into four groups, one control group and three treated groups with different doses of methotrexate. Each group consisted of fifteen mice. The control group was injected intraperitonealy with 1ml/kg b.wt. distilled water, while treated group (1) was intraperitoneally injected with 2.5 mg / kg b. wt., single dose at the first day of the experiment and sacrificed by cervical dislocation after 24, 48 and 72 hour of treatment, treated group (2) was intraperitoneally injected with methotrexate 5 mg / kg b.wt., single dose at the first day of the experiment and sacrificed by cervical dislocation after 24, 48 and 72 hour of treatment and treated group (3) was intraperitoneally injected with methotrexate 10 mg / kg b.wt., single dose at the first day of experiment and sacrificed by cervical dislocation after 24, 48 and 72 hour of treatment.
The present study showed that the normal mouse chromosomes are 40 telocentric chromosomes. They are divided into five distinct groups; each contains the chromosomes of nearly equal lengths.
The current results showed that the tested doses of methotrexate induced structural and numerical chromosomal aberrations in male albino mice bone marrow cells which were highly significant increased (P< 0.001) by dose and time. Structural aberrations were chromosomal and chromatid gaps, fragments, centromeric attenuation, deletion, centric fusion, ring formation, end to end association and beaded chromosomes. Also, methotrexate treatment caused numerical aberration in the form of polyploidy.
Other data
| Title | GENOTOXIC EFFECT OF METHOTREXATE ON BONE MARROW CHROMOSOMES AND DNA OF MALE ALBINO MICE Mus musculus | Other Titles | تأثير السمية الوراثية للميثوتركسيت على كروموسومات نخاع العظم والحمض النووى د ن أ فى ذكور الفئران المهقاء ماس مسكيولس | Authors | Sally Ramadan Gabr Eid El-Ashry | Issue Date | 2015 |
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