Cytotoxicity and flow rate of Triple Antibiotic Paste versus Propolis as an intracanal medicament. ( An In-Vitro study)
Hend Mohammed Hamdy Ismail;
Abstract
The aim of the study was to compare the cytotoxicity and the flow properties of Triple antibiotic paste versus propolis as an intracanal medicament.
Pastes of Triple Antibiotic pastes were prepared and and sterilised by ethylene oxide gas and soaked in E-MEM for 7 days .Samples were placed in a dark container at 37 °C and 100%.
Cytotoxicity was evaluated using the MTT assay at 24 , 48 and 72 hours, IC50 values were calculated as well as the therapeutic dose cytotoxicity values. Flow properties were evaluated by putting the pastes between two parallel glass slabs and inserting a weight of 500grams on the parallel slabs , diameter of the circles formed were measured and calculated using a vernier ruler. Data was calculated and statististically analysed.
MTT results showed insignificant difference between Triple antibiotic paste when evaluated at 11 different doses .
When IC50values were calculated from readings obtained from MTT assay results showed statistical significant differences.
When therapeutic values obtained from MTT assay readings were evaluated results showed significant statistical differences ( P value : 0.0008) between both propolis and Triple Antibiotic Paste with highest viable cell percentage to triple antibiotic paste with 87.53% vitality at 48 hours followed by 76.63% vitality at 72 hours of TAP followed by 76.32 vitality of TAP at 24 hours then Propolis with a low cell vitality of 34.21% at 72 hours then 32.54% vitality at 48 hours and the least cell vitality percentage was found for Propolis at 24 hours 20.5% suggesting it to have the most cytotoxic action when therapeutic dose was used .
Pastes of Triple Antibiotic pastes were prepared and and sterilised by ethylene oxide gas and soaked in E-MEM for 7 days .Samples were placed in a dark container at 37 °C and 100%.
Cytotoxicity was evaluated using the MTT assay at 24 , 48 and 72 hours, IC50 values were calculated as well as the therapeutic dose cytotoxicity values. Flow properties were evaluated by putting the pastes between two parallel glass slabs and inserting a weight of 500grams on the parallel slabs , diameter of the circles formed were measured and calculated using a vernier ruler. Data was calculated and statististically analysed.
MTT results showed insignificant difference between Triple antibiotic paste when evaluated at 11 different doses .
When IC50values were calculated from readings obtained from MTT assay results showed statistical significant differences.
When therapeutic values obtained from MTT assay readings were evaluated results showed significant statistical differences ( P value : 0.0008) between both propolis and Triple Antibiotic Paste with highest viable cell percentage to triple antibiotic paste with 87.53% vitality at 48 hours followed by 76.63% vitality at 72 hours of TAP followed by 76.32 vitality of TAP at 24 hours then Propolis with a low cell vitality of 34.21% at 72 hours then 32.54% vitality at 48 hours and the least cell vitality percentage was found for Propolis at 24 hours 20.5% suggesting it to have the most cytotoxic action when therapeutic dose was used .
Other data
| Title | Cytotoxicity and flow rate of Triple Antibiotic Paste versus Propolis as an intracanal medicament. ( An In-Vitro study) | Other Titles | تأثيرسميه و صفة تدفق صمغ النحل ومعجون ثلاثي المضادات علي الخلايا الحيويه | Authors | Hend Mohammed Hamdy Ismail | Issue Date | 2015 |
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