The Rellattiionshiip bettween IIL28B Genottypes and Hepattoma iin Postt HCV Ciirrhotiic Patiientts

Abstract

Hepatitis C continues to be a major public health problem affecting approximately 3% of the global population (World Health Organization, 2007), estimated HCV prevalence in Egypt range from 11% to 14% with 8 to 10 million having anti-HCV and 5 to 7 million having active infections. HCV prevalence is directly associated with the amount of intravenous tartar emetic used to control schistosomiasis (Mohamed, 2004), 10% -20% of those who are chronically infected with chronic hepatitis C will progress to cirrhosis and 5% will develop hepatocellular carcinoma, by which it has became a worldwide concern to find several ways in early detection and treating such dangerous leathal disease, TheIL28B genotype have been shown to play an important role in HCV treatment outcomes and spontaneous clearance of the virus (Kelly et al., 2011). So the current study aimed to find out whether there is a relation between IL28B genotypes and development of hepatoma and hepatic decompensation, We aimed To determine the relationship between the IL28B genotype and hepatocarcinogenesis in Egyptian patients infected by HCV genotype 4 by by genotyping 40(20 hepatoma cases, 20 decompensated liver cases) patients with age ranging between from45 to 72 years from both genders with chronic hepatitis C in outpatient clinic in Ain Shams University hospital. All patients were subjected to Medical history, complete blood count, liver profile, serum kidney function tests, viral hepatitis markers, alpha fetoprotein, abdominal (U/S) and abdominal Triphasic spiralCT for hepatic focal lesion. HCV genotype, and IL28b genotyping by sequencing mechanism (Taq Man® Real- Time PCR) to detect type of IL28b polymorphism and patients divided according to their genotype into 3 groups. Group 1: CC favorable Group 2: CT unfavorable Group 3: TT. Unfavorable gene. This study reported the following: In our study (40 cases) As regard gene polymorphism, it was CC in 11 cases (27.5%), CT in 10 cases (25.0%) and TT in 19 cases (47.5%). Hepatoma (20 cases) the distribution was CC, CT and TT in 15.0%, 30.0% and 55.0% respectively; while in in decompensated cases 20 case, the distribution was CC, CT and TT in 40.0%, 20.0% and 40.0% respectively, that may explain the relationship between IL28B genotypes and occurrence of (H.C.C) and (LC) liver cell failure, in patients with T allele rather than patients who have C allele (table 10and Fig.10).