IN VITRO PROPAGATION AND PRODUCTION OF SOME NATURAL ANTIMICROBIAL COMPOUNDS FROM MORINGA OLEIFERA TREES USING TISSUE CULTURE TECHNIQUES

Abstract

This study was carried in the Plant Tissue Culture Lab., Hort. Res. Inst., Aric. Res. Center, Giza during the periods of 2010 – 2014 aiming to propagate and produce some biocompounds of Moringa oleifera in vitro by using different treatments and consequently the propagation ability of such explants, during the four seasons of investigation. The data obtained were statically analyzed and results may be summarized as follows: 1. In vitro propagation 1.1. Establishment stage 1.1.1. Effect of sterilization treatments Shoot tip explants • Using HgCl2 at 0.1 % for 7 min resulting the peak value (100 %) of disinfection rate of Moringa oleifera shoot tip explants. • The highest survival rates of shoot tip explants (60 % and 80 %) resulted in case of using 0.1 % HgCl2 for 5 or 7 min, respectively. Stem node explants • Applying either Clorox at 10 % or at 20 % for 15 min, or Clorox at 10 % followed by HgCl2 at 0.2 % for 15 min were resulted in the peak value of aseptic nodal explants cultures (100 % decontamination). • The survival potentiality of stem nodal explants was not significantly influenced by the types and concentrations (Clorox at 10 or 20 % and HgCl2 at 0.1 or 0.2 %) when they used individually or in a combination form between them.

1.2. Multiplication stage