Role of blaZ Gene in Staphylococcus Aureus Resistance to Penicillin
Mostafa Bakri Hulo;
Abstract
The spread of penicillinase-producingStaphylococcalwas known for many years as a global issue. The emergence of resistance to penicillin made the treatment for infections caused by these penicillinase -producing Staphylococcal strains very limited.
The most important mechanism of resistance to Penicillin is the production of beta lactamase by Staphylococcus aureus.
Rapid and accurate detection methods for of beta lactamase producing Staphylococcus aureus is very essential to implement appropriate choice of treatment and antibiotic choose.
The aim of this work is to identify prevalence ofS. aureusresistance to penicillin and to evaluate the nitrocefin reaction in detecting β-lactamse production in S. aureus isolates. This would be compared to detection of blaZ- gene presence by real time PCR in S. aureus isolates obtained from various clinical samples.
The study included 100 isolates of S. aureus that were recovered from various clinical samples submitted to the main Microbiology Laboratory for routine culture and susceptibility testing in the period from January to April 2016.
All isolates were subjected to Antimicrobial susceptibility using cefoxitin and penicillin then Nitrocefen test was made for all the samples blaZ gene was detected by real time PCR .
The most important mechanism of resistance to Penicillin is the production of beta lactamase by Staphylococcus aureus.
Rapid and accurate detection methods for of beta lactamase producing Staphylococcus aureus is very essential to implement appropriate choice of treatment and antibiotic choose.
The aim of this work is to identify prevalence ofS. aureusresistance to penicillin and to evaluate the nitrocefin reaction in detecting β-lactamse production in S. aureus isolates. This would be compared to detection of blaZ- gene presence by real time PCR in S. aureus isolates obtained from various clinical samples.
The study included 100 isolates of S. aureus that were recovered from various clinical samples submitted to the main Microbiology Laboratory for routine culture and susceptibility testing in the period from January to April 2016.
All isolates were subjected to Antimicrobial susceptibility using cefoxitin and penicillin then Nitrocefen test was made for all the samples blaZ gene was detected by real time PCR .
Other data
| Title | Role of blaZ Gene in Staphylococcus Aureus Resistance to Penicillin | Other Titles | دور الجين (gene (BlaZفي مقاومة المكورات العنقودية الذهبية للبنسلين | Authors | Mostafa Bakri Hulo | Issue Date | 2017 |
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