CLINICAL DIAGNOSIS OF OCULAR INFECTION OF CHLAMYDIA TRACHOMATIS BY POLYMERASE CHAIN REACTION, MOLECULAR APPROACH
Mohamad Sharaf El-Din Zaky Badr;
Abstract
Trachoma is an infectious preventable blinding disease endemic in Egypt since pre-historic time. In order to provide suitable preventive measures against trachoma, the epidemiology of the disease has to be clear.
The aim of the present work is to develop a new polymerase chain reaction (PCR) technique for early, accurate and fast diagnosis of C. trachomatis.
The present study included 48 patients belonged to different age, sex, type of work, and environment categories. The studied cases included 25 females and 23 males. A full clinical sheet was taken from all subjects. Conjunctival swabs were collected for detection of C. trachomatis DNA of the MOMP and plasmid genes by PCR.
The study also involved the evaluation of six different methods of DNA extraction from conjunctival swabs of trachoma patients to find out the sensitive, simple, easy, and least expensive method for routine diagnosis of trachoma. These methods were glass fibre affinity, freeze-thaw, ionic detergent, non-ionic detergent, boiling and phenol-chloroform extraction methods.
The aim of the present work is to develop a new polymerase chain reaction (PCR) technique for early, accurate and fast diagnosis of C. trachomatis.
The present study included 48 patients belonged to different age, sex, type of work, and environment categories. The studied cases included 25 females and 23 males. A full clinical sheet was taken from all subjects. Conjunctival swabs were collected for detection of C. trachomatis DNA of the MOMP and plasmid genes by PCR.
The study also involved the evaluation of six different methods of DNA extraction from conjunctival swabs of trachoma patients to find out the sensitive, simple, easy, and least expensive method for routine diagnosis of trachoma. These methods were glass fibre affinity, freeze-thaw, ionic detergent, non-ionic detergent, boiling and phenol-chloroform extraction methods.
Other data
| Title | CLINICAL DIAGNOSIS OF OCULAR INFECTION OF CHLAMYDIA TRACHOMATIS BY POLYMERASE CHAIN REACTION, MOLECULAR APPROACH | Other Titles | التشخيص الاكلينيكى للكلاميديا تراكومانيس باستخدام تفاعل البلمرة المتسلسل (مدخل الوراثة الجزيئية ) | Authors | Mohamad Sharaf El-Din Zaky Badr | Issue Date | 2000 |
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