Effect of Lipoic acid and Squalene on Cyclophosphamide-Induced Oxidative Stress in Rats
Ayat Refaat Hussein Abdel-Moaty Omar;
Abstract
The present study aimed to evaluate the role of DL-alpha-lipoic acid (LA) and squalene (SQ) on oxidative cardiac, testicular and urotoxic damage induced by cyclophosphamide (CP). Male Wistar rats were divided into four groups; three groups received a single intraperitoneal (i.p.) injection of CP (200 mg/kg body wt.), and two ofthese groups received either LA (35 mg/kg body wt.) or SQ (0.4 ml/rat) orally for 7 days before and 7 days after CP injection. A vehicle-treated control group was also included. Oxidative damage was observed by decreased serum total antioxidant capacity (TAC) level and abnormal alterations in glutathione peroxidase (GPx) and glutathione reductase (GR) activities, levels of glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO) and calcium in the heart, testes and urinary bladder of CP-administered rats. Cardiac marker enzyme activities; creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) showed severe declines whereas testicular markers; sorbitol dehydrogenase (SDH), y-glutamyl transpeptidase (GGT), acid and alkaline phosphatases (ACP and ALP), serum testosterone (T) level and haemoglobin (Hb) absorbance were abnormal. Histopathological observations were also altered. These CP-induced pathological alterations were attenuated by treatment with LA or SQ. These findings highlight the efficacy of LA and SQ as cytoprotectants in CP-induced toxicity.
Other data
Title | Effect of Lipoic acid and Squalene on Cyclophosphamide-Induced Oxidative Stress in Rats | Other Titles | تأثير حمض الليبويك والسكوالين ضد الاجهاد الاوكسيدي الناتج من السيكلوفوسفاميد في الجرزان | Authors | Ayat Refaat Hussein Abdel-Moaty Omar | Keywords | : Cyclophosphamide, Lipoic acid, Squalene, Heart, Testes, Urinary bladder, Rats, Oxidative stress. | Issue Date | 2010 |
Attached Files
File | Size | Format | |
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Ayat Refaat Hussein Abdel-Moaty Omar.pdf | 1.4 MB | Adobe PDF | View/Open |
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