Study of Some Lymphocyte Subsets in Children with Newly Diagnosed Primary Immune Thrombocytopenia
Amna Helmy Awad;
Abstract
SUMMARY
T
he aim of the study was to explore the clinical significance of some T-lymphocyte subsets in the peripheral blood of children with newly diagnosed immune thrombocytopenia.
Current first line of treatment for patients with ITP is corticosteroids and intravenous immunoglobulin, but the efficacy of steroids is limited. Moreover the association between lymphocyte subsets and therapeutic outcome is rarely reported. In this study, we try to analyze the correlation between lymphocyte subsets and therapeutic outcome after treatment with corticosteroids.
CD3, CD4, CD8, in the peripheral blood of 30 newly diagnosed ITP children were detected by flowcytometry. ITP patients were treated by corticosteroids and therapeutic response was evaluated after 4 months.
It turned out that among 30 ITP patients 18 cases shows complete response to therapy, 4 cases showed loss of complete response to therapy, 7 cases exhibited loss of response and 1 case had response.
The mean age was 9.9 years with minimum 2 years and maximum 9 years, it was found that the younger age of patients was associated with complete response to therapy and it was clear that mean age of 6.9 years was associated with worse outcomes .
It was observed that the TLC in CR group was higher compared to loss of R and R group yet it was not statistically significant.
Level of CD3, CD4, CD8 were compared to normal range and we found that CD3 was above average 10% below average in 20% o and within average in 70% of studied group.CD4 was below average in 30% and within average in 70% of studied group.CD8 was above average in 10%, below average in 23.3%, and within average in 66.7% of studied group. CD4/CD8 ratio was above average in 3.3%, below average in 20% and within average in 76.7% of studied group.
There was significant positive correlation between total leucocytic count at diagnosis and CD3,.And significant positive correlation between platelets after follow up and CD4 And high significant positive correlation between lymphocyte at diagnosis and CD3, CD4,CD8. And high negative significant correlation between lymphocytes after follow up and CD4/CD8 ratio.
There was no significant relation between CD3+, CD8+,CD4+ T lymphocytes and response to therapy, however it was observed that there is increase in expression of CD4+ T lymphocytes among the complete response compared with the response group but this was not significant, which may need further evaluation.
Understanding the role of T cell subsets will permit a better control of autoimmunity.
T
he aim of the study was to explore the clinical significance of some T-lymphocyte subsets in the peripheral blood of children with newly diagnosed immune thrombocytopenia.
Current first line of treatment for patients with ITP is corticosteroids and intravenous immunoglobulin, but the efficacy of steroids is limited. Moreover the association between lymphocyte subsets and therapeutic outcome is rarely reported. In this study, we try to analyze the correlation between lymphocyte subsets and therapeutic outcome after treatment with corticosteroids.
CD3, CD4, CD8, in the peripheral blood of 30 newly diagnosed ITP children were detected by flowcytometry. ITP patients were treated by corticosteroids and therapeutic response was evaluated after 4 months.
It turned out that among 30 ITP patients 18 cases shows complete response to therapy, 4 cases showed loss of complete response to therapy, 7 cases exhibited loss of response and 1 case had response.
The mean age was 9.9 years with minimum 2 years and maximum 9 years, it was found that the younger age of patients was associated with complete response to therapy and it was clear that mean age of 6.9 years was associated with worse outcomes .
It was observed that the TLC in CR group was higher compared to loss of R and R group yet it was not statistically significant.
Level of CD3, CD4, CD8 were compared to normal range and we found that CD3 was above average 10% below average in 20% o and within average in 70% of studied group.CD4 was below average in 30% and within average in 70% of studied group.CD8 was above average in 10%, below average in 23.3%, and within average in 66.7% of studied group. CD4/CD8 ratio was above average in 3.3%, below average in 20% and within average in 76.7% of studied group.
There was significant positive correlation between total leucocytic count at diagnosis and CD3,.And significant positive correlation between platelets after follow up and CD4 And high significant positive correlation between lymphocyte at diagnosis and CD3, CD4,CD8. And high negative significant correlation between lymphocytes after follow up and CD4/CD8 ratio.
There was no significant relation between CD3+, CD8+,CD4+ T lymphocytes and response to therapy, however it was observed that there is increase in expression of CD4+ T lymphocytes among the complete response compared with the response group but this was not significant, which may need further evaluation.
Understanding the role of T cell subsets will permit a better control of autoimmunity.
Other data
| Title | Study of Some Lymphocyte Subsets in Children with Newly Diagnosed Primary Immune Thrombocytopenia | Other Titles | دراسه لبعض المجموعات الفرعية للخلايا الليمفاوية تى فى الاطفال حديثي الاصابه بنقص الصفائح المناعي الأولى | Authors | Amna Helmy Awad | Issue Date | 2017 |
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