MOLECULAR MARKERS ASSOCIATED WITH THE EXPRESSION OF PATHOGENICITY GENES OF RHIZOCTONIA SOLANI CAUSING FABA BEAN ROOT ROT
MAHA HELMY MOHAMED HASSAN;
Abstract
Root rot disease of faba bean (Vicia faba L.) is a widespread disease under greenhouse and field conditions. The causal organism Rhizoctonia solani is causes agriculturally significant diseases on an enormous range of crop species. This research aimed to identify the most important genes that up regulate in Rhizoctonia solani during pathogenesis in order to find out a new approach to control Rhizoctonia disease on faba bean.
The results could be summarized as follows:
1- One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants suffering from root-rot and stem canker. These isolates were collected from different governorates (El-Kaliobia, El-Sharkia, and El-Giza) and from different plant stages. Rhizoctonia isolates were divided into two groups i.e. binucleate and polynucleate. Percentage of binucleate isolates reached 64.88 %, while polynucleate isolates reached 35.11 %.
2- According to the morphological features of Rhizoctonia isolates grown on Petri dishes contained potato sucrose agar medium, all isolates were divided into twelve groups. Several isolates (twenty one isolates) were randomly chosen for pathogenicity test procedure. Chosen isolates were varied according to their aggressiveness on peeled germinated faba bean seeds. The most aggressive isolate belong to polynucleate Rhizoctonia was identified either on morphological or on molecular level. According to its morphological features, this isolate was identified as Rhizoctonia solani. Thereafter, this isolate was additionally identified on molecular level, the isolate was identified as Rhizoctonia solani AG4-HGI by using specific primers of ITS regions of rDNA.
3- Anastomosis test was carried between accurately identified isolate and eleven isolates belong to polynucleate groups. All isolates were perfectly fused. This result indicated that the eleven chosen isolates belonging to the same AG group i.e. AG4-HGI.
The results could be summarized as follows:
1- One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants suffering from root-rot and stem canker. These isolates were collected from different governorates (El-Kaliobia, El-Sharkia, and El-Giza) and from different plant stages. Rhizoctonia isolates were divided into two groups i.e. binucleate and polynucleate. Percentage of binucleate isolates reached 64.88 %, while polynucleate isolates reached 35.11 %.
2- According to the morphological features of Rhizoctonia isolates grown on Petri dishes contained potato sucrose agar medium, all isolates were divided into twelve groups. Several isolates (twenty one isolates) were randomly chosen for pathogenicity test procedure. Chosen isolates were varied according to their aggressiveness on peeled germinated faba bean seeds. The most aggressive isolate belong to polynucleate Rhizoctonia was identified either on morphological or on molecular level. According to its morphological features, this isolate was identified as Rhizoctonia solani. Thereafter, this isolate was additionally identified on molecular level, the isolate was identified as Rhizoctonia solani AG4-HGI by using specific primers of ITS regions of rDNA.
3- Anastomosis test was carried between accurately identified isolate and eleven isolates belong to polynucleate groups. All isolates were perfectly fused. This result indicated that the eleven chosen isolates belonging to the same AG group i.e. AG4-HGI.
Other data
| Title | MOLECULAR MARKERS ASSOCIATED WITH THE EXPRESSION OF PATHOGENICITY GENES OF RHIZOCTONIA SOLANI CAUSING FABA BEAN ROOT ROT | Other Titles | العلامات الجزيئية المصاحبة لتعبير جينات الإمراض لفطر Rhizoctonia solani المسبب لعفن الجذور في الفول البلدي | Authors | MAHA HELMY MOHAMED HASSAN | Issue Date | 2015 |
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