Evaluation of Chemopreventive Activity of Curcumin Lipoiate as an Inhibitor of Leukemia using Nanotechnology

Abstract

One of the most dangerous types of cancer in Egypt is leukemia which is predominant in children more than adult. The present study aimed to evaluate the chemopreventive activity of curcumin lipoiate on tumor progression, chemotherapeutic efficacy and hematopoiesis in the peripheral blood either alone or with nanoparticle (ZnO) and on 7,12- dimethylbenz (a) anthrathene (DMBA) induced leukemia intravenous in female wistar rats. The level of β2 microglobulin in serum and CBC profile in addition to the activities of the most important free radical scavengers of the antioxidant defense system in plasma including ; reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase, as well as malondialdehyde content which is considered an indicator of lipid peroxidation were estimated. Liver function & kidney function tests were also determined for all groups. Cytopathological examination of peripheral blood of each group was also performed. The result of the present study revealed that, curcumin lipoiate conjugated with ZnO nanoparticles, is more potent antitumor effect than in free form, as it normalizes most of the investigated parameters. In conclusion, this results proved that, the conjugation form resulted in super-additive inhibitor effects on leukemic cells and this was well appreciated with the cytopathological alteration findings in the treated groups, these findings suggest that curcumin lipoiate with ZnO play an important role in prevention of leukemia.