BIOCHEMICAL AND MOLECULAR RESPONSES ASSOCIATED WITH UV-INDUCED RESISTANCE IN PLANT
MOHAMED MAHMOUD ABOUL FOTOUH MESALHI;
Abstract
Due to the stratospheric ozone depletion, intense care was paid to investigate the adverse effects of increasing solar UV-B on plants and different living organisms. However, many studies proved that application of low doses of UV on plants enhanced plant tolerance to other stresses. Therefore, the impact of UV-C seed treatment on the induction of adaptive mechanisms in grown plants under stressful conditions was studied.
Salinity stress is considered to be one of the limiting factors for agricultural production. Thus, salinity stress was chosen as a challenge for UV seed treatment. Accordingly, dry or germinated seeds of cucumber (moderate sensitive to salinity stress) and green bean (salt stress-sensitive) were exposed to UV-C radiation (254 nm) for durations of 7, 15, 30 and 60 min, then seeds were stored in dark to minimize any photoreactivation processes. After emergence of the first true leaf, cucumber and green bean seedlings of each UV treatment group were divided into two subgroups: the first group was irrigated with Hoagland solution and the other group was subjected to salinity stress (50 mM NaCl + Hoagland solution). Afterwards, cucumber plants (24 days-old) and green bean plants (45 days-old) were harvested and separated to shoots and roots.
In order to investigate the ability of UV seed treatment to enhance salinity stress tolerance in both plants, shoots and roots fresh and dry weights were measured. As well, root to shoot ratios were determined. Also, biochemical changes in leaves and roots were studied by determination of : lipid peroxidation levels (MDA), proline concentration, total phenols concentration and the activities of defensive enzymes i.e. phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (G-POD), ascorbate peroxidase (APX) and polyphenol oxidase (PPO). Moreover, electrophoretic pattern of peroxidase isozymes in leaf samples which exhibited the maximum peroxidase activity was also examined.
Salinity stress is considered to be one of the limiting factors for agricultural production. Thus, salinity stress was chosen as a challenge for UV seed treatment. Accordingly, dry or germinated seeds of cucumber (moderate sensitive to salinity stress) and green bean (salt stress-sensitive) were exposed to UV-C radiation (254 nm) for durations of 7, 15, 30 and 60 min, then seeds were stored in dark to minimize any photoreactivation processes. After emergence of the first true leaf, cucumber and green bean seedlings of each UV treatment group were divided into two subgroups: the first group was irrigated with Hoagland solution and the other group was subjected to salinity stress (50 mM NaCl + Hoagland solution). Afterwards, cucumber plants (24 days-old) and green bean plants (45 days-old) were harvested and separated to shoots and roots.
In order to investigate the ability of UV seed treatment to enhance salinity stress tolerance in both plants, shoots and roots fresh and dry weights were measured. As well, root to shoot ratios were determined. Also, biochemical changes in leaves and roots were studied by determination of : lipid peroxidation levels (MDA), proline concentration, total phenols concentration and the activities of defensive enzymes i.e. phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (G-POD), ascorbate peroxidase (APX) and polyphenol oxidase (PPO). Moreover, electrophoretic pattern of peroxidase isozymes in leaf samples which exhibited the maximum peroxidase activity was also examined.
Other data
| Title | BIOCHEMICAL AND MOLECULAR RESPONSES ASSOCIATED WITH UV-INDUCED RESISTANCE IN PLANT | Other Titles | الاستجابات البيوكيميائية و الجزيئية المصاحبة للمقاومة المستحثة بالأشعة فوق البنفسجية فى النبات | Authors | MOHAMED MAHMOUD ABOUL FOTOUH MESALHI | Issue Date | 2014 |
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