New Nano Optical Sensors for the assessment of Some Materials of biological Activities in Human Body.
Ibrahim Atfi Ibrahim;
Abstract
New Nano Optical Sensors for the assessment of Some Materials of biological Activities in Human Body.
The study presented in this thesis include development and the combination between some techniques and technologies such as Analytical chemistry, Biochemistry, Photochemistry and Nanotechnology for the determination of some active drugs components (Group of Tetracyclines) and human enzymes such as alpha fucosidase.
The thesis is divided into three chapters:
Chapter one:-
Including a general introduction on lanthanides, optical sensors and other methods used for determination of alpha fucosidase Enzyme.
Chapter two:-
Studying the five optical sensor Eu3+-Tetracyclines Complexes for assessment of alpha –fucosidase enzyme activity in human liver and select the best one at optimum condition suitable for the determination of alpha fucosidase activity in some real serum samples. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated on 5-Complexes of Europium -Tetracyclines.
Chapter three:-
Describes novel, simple, sensitive and selective spectrofluorimetric method for the determination of Tetracyclines in pharmaceutical tablets, serum samples using optical sensor Eu3+-Tetracyclines. The product of Reaction Alpha fucosidase with its substrate 2CNP can remarkably quench the luminescence intensity of Tetracyclines complex in ACN at pH 8.4 and λex= 410nm( energy transfer from Probe to enzyme)The quenching of luminescence intensity peak of Eu3+-Tetracyclines complex at λem =618 nm is proportional to the concentration of 2-CNP and Eu3+-Tetracyclines complex nano
The study presented in this thesis include development and the combination between some techniques and technologies such as Analytical chemistry, Biochemistry, Photochemistry and Nanotechnology for the determination of some active drugs components (Group of Tetracyclines) and human enzymes such as alpha fucosidase.
The thesis is divided into three chapters:
Chapter one:-
Including a general introduction on lanthanides, optical sensors and other methods used for determination of alpha fucosidase Enzyme.
Chapter two:-
Studying the five optical sensor Eu3+-Tetracyclines Complexes for assessment of alpha –fucosidase enzyme activity in human liver and select the best one at optimum condition suitable for the determination of alpha fucosidase activity in some real serum samples. The optimal experimental conditions such as solvent, pH and molar ratio corresponding to maximum luminescence intensity were investigated on 5-Complexes of Europium -Tetracyclines.
Chapter three:-
Describes novel, simple, sensitive and selective spectrofluorimetric method for the determination of Tetracyclines in pharmaceutical tablets, serum samples using optical sensor Eu3+-Tetracyclines. The product of Reaction Alpha fucosidase with its substrate 2CNP can remarkably quench the luminescence intensity of Tetracyclines complex in ACN at pH 8.4 and λex= 410nm( energy transfer from Probe to enzyme)The quenching of luminescence intensity peak of Eu3+-Tetracyclines complex at λem =618 nm is proportional to the concentration of 2-CNP and Eu3+-Tetracyclines complex nano
Other data
| Title | New Nano Optical Sensors for the assessment of Some Materials of biological Activities in Human Body. | Authors | Ibrahim Atfi Ibrahim | Issue Date | 2017 |
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