NEW BIOAPPROACH FOR THE PRODUCTION OF THE MANURE FROM ORGANIC WASTES AND THEIR APPLICATION ON DESERT SOIL

Amal Mohamed Omar Salem


Abstract


Composting depends on biological process to decompose organic compounds of solid waste. Composting of rice straw or garbage supplemented with chemical accelerator, sheep manure and mixture of cellulolytic microorganisms was conducated and mixture of Azotobacter • chroococcum and P-solubilizing bacteria as biofertilizer was added to half amount of compost to give qualified one. The enhanced use of bioinoculum in combination with chemical amendments accelerated the compost maturity and shorted the usual period of composting. Cellulolytic bacteria and fungi isolates were isolated from different sources. The isolates which gave faster positive results for their cellulase activity were identified as Micrococcus luteus , Kocuria rosea and Stachybortys sp. These strains were subjected to further environmental and nutritional requirements. The optimum conditions for maximizing enzyme activity was addition of CMC as the most active carbon source for growth proliferation and enzyme activity for all cellulolytic strains at 1% concentration. Also, addition of yeast as nitrogen source at 0.8 giL for bacterial strain ; MnS04 (0.5 giL) for Micrococcus luteus and CaCh 0.5 g/1 forK. rosea as metallic ions and incubation period at 45°C and pH 7.O.for 1 day and 4 days, respectively. For Stachybotrys sp., addition of peptone 2.2 giL as N- source and CoS04 (0.5 giL) as metallic ions and incubation period for 5 days at 30°C and pH 5.5 gave the maximum enzyme activity. The laboratory results could be summarized as follow: I. The highest diameter of CMC degradation zone was 3.2 em, 2.8 em, 2.4 em for Stachybotrys sp., M luteus andMrosea, respectively.


Other data

Other Titles التقنيات الحيوية الحديثة لأنتاج السماد من المخلفات العضوية واستخداماتها تحت ظروف الأراضى الصحراوية
Issue Date 2006
URI http://research.asu.edu.eg/handle/12345678/47581


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