Pathological study on green tea effect on experimentally infected chickens with highly pathogenic strain of avian influenza virus (H5N1)
Marwa Ali Abd-Elmagid Hassan;
Abstract
Abstract
Avian influenza virus; one of the most important zoonotic diseases that’s not only pose a
threat to the poultry industry but also high risk to the humans and environment. Although; many
efforts are made to control it to reduce its risk globally mainly through stamping out or
vaccination particularly in Egypt, it’s still spreading and become endemic in Egypt since 2008.
Therefore, recent attention has been directed to alternative medicine because of its many health
benefits and antiviral effects. In this study we evaluated the antiviral efficacy of green tea
extract on highly pathogenic avian influenza virus (H5N1). Different concentrations of
methanolic green tea extract were inoculated into vero cell line and to ECE to study its
cytotoxicity and antiviral effect in-vitro. 120 one day old SPF chicks were divided into 8 groups
(15 birds/group); group (A) negative control, group (B) GTE control, group (C) positive control,
group (D) supplied with GTE from beginning of the experiment, group (E) supplied with GTE
4hs before challenge, group (F) supplied with GTE 24hs post challenge, group (G) vaccinated
with inactivated vaccine (Re-1) on the 7th day old and group (H) supplied by GTE from
beginning of the experiment and vaccinated. GTE used 5gm/liter in drinking water. The
challenge was applied on the 28th day old using HPAI (H5N1)10
6
EID50/100µl/bird through
I/N rout. Our results revealed desirable effect in-vitro by marked decreasing in viral cytopathic
effect on treated vero cells as well as decreasing the hemagglutinating titer in tested allantoic
fluid of treated ECE group. Obvious antiviral efficacy of GTE was detected in-vivo including
decreases in viral shedding of treated groups; particularly in vaccinated treated one, clear
histopathological improvement of lung of treated groups which confirmed by variable detection
of viral antigen by immunohistochemistry and morphometric analysis.
Avian influenza virus; one of the most important zoonotic diseases that’s not only pose a
threat to the poultry industry but also high risk to the humans and environment. Although; many
efforts are made to control it to reduce its risk globally mainly through stamping out or
vaccination particularly in Egypt, it’s still spreading and become endemic in Egypt since 2008.
Therefore, recent attention has been directed to alternative medicine because of its many health
benefits and antiviral effects. In this study we evaluated the antiviral efficacy of green tea
extract on highly pathogenic avian influenza virus (H5N1). Different concentrations of
methanolic green tea extract were inoculated into vero cell line and to ECE to study its
cytotoxicity and antiviral effect in-vitro. 120 one day old SPF chicks were divided into 8 groups
(15 birds/group); group (A) negative control, group (B) GTE control, group (C) positive control,
group (D) supplied with GTE from beginning of the experiment, group (E) supplied with GTE
4hs before challenge, group (F) supplied with GTE 24hs post challenge, group (G) vaccinated
with inactivated vaccine (Re-1) on the 7th day old and group (H) supplied by GTE from
beginning of the experiment and vaccinated. GTE used 5gm/liter in drinking water. The
challenge was applied on the 28th day old using HPAI (H5N1)10
6
EID50/100µl/bird through
I/N rout. Our results revealed desirable effect in-vitro by marked decreasing in viral cytopathic
effect on treated vero cells as well as decreasing the hemagglutinating titer in tested allantoic
fluid of treated ECE group. Obvious antiviral efficacy of GTE was detected in-vivo including
decreases in viral shedding of treated groups; particularly in vaccinated treated one, clear
histopathological improvement of lung of treated groups which confirmed by variable detection
of viral antigen by immunohistochemistry and morphometric analysis.
Other data
| Title | Pathological study on green tea effect on experimentally infected chickens with highly pathogenic strain of avian influenza virus (H5N1) | Authors | Marwa Ali Abd-Elmagid Hassan | Issue Date | 2018 |
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