MODULATION OF IN-VITRO COLONY FORMATION OF PHILADELPHIA­ POSITIVE CELLS BY ANTISENSE OLIGODEOXYNUCLEOTIDES IN CML

Samah M. Abd EL Hameed;

Abstract


CML is a prototype of a disease in which the molecular oncogenesis is known, . caused by the Philadelphia chromosome. The introduction of antisense oligodeoxynucleotides (AS-ODNs) against the transcribted mRNA of b3a2 and b2a2 breakpoints leads to suppresion of translation of the abnormal p210 tyrosine kinase protein. Comparing b2a2 AS-ODNs inhibition ratio to both b3a2 and TAT AS-ODNs inhibition ratios showed a highly significant statistical difference. Also there was a significant
•negative statistical correlation between AS-ODNs inhibition

ratios• and both basophil percent and LAP score in chronic phase CML thus AS-ODNs can be used to purge autologous stem cells to reduce malignant cells before its transplantation in chronic phase CML.


Other data

Title MODULATION OF IN-VITRO COLONY FORMATION OF PHILADELPHIA­ POSITIVE CELLS BY ANTISENSE OLIGODEOXYNUCLEOTIDES IN CML
Other Titles دراسة تأثير سلسلة الوحدات العاكسة لشفرة الحامض النووى على المستعمرات المكونة من الخلايا الإيجابية لفيلاديلفيا كروموزوم فى سرطان الدم الميلويدى
Authors Samah M. Abd EL Hameed
Issue Date 2001

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