Isolation and identification of Enterococcus spp from chicken
Shimaa Hamdy Okasha Ahmed;
Abstract
1. Thirty seven liver samples and thirty seven spleen samples (total =74 samples) were collected from different types of chicken broilers and layers for investigation to Enterococcus isolation between sep,2013 and Jan,2014.
2. All samples were cultured under sterile conditions on Modified Edward,s medium which is a selective medium for isolation of Enterococcus.
3. Suspected Enterococcus colonies were identified biochemically and 14 isolates were identified as Enterococcus.
4. Biochemically identified Enterococci isolates were confirmed by PCR .
5. PCR was performed to identify E.faecalis and E.faecium by using specific primer for each. Six Isolates were E. faecalis and eight isolates were E. faecium .
6. All isolates were tested for antibiotic sensitivity test; Results revealed that 14.3 % of isolates were sensitive to penicillin G , 57.2 % of the isolates were
sensitive to gentamicin, 7.1% were sensitive to chloramphenicol, 21.4% were sensitive to vancomycin and 28.5 % were sensitive to ciprofloxacin.
On the other hand, 78.6 % of isolates were resistant to erythromycin, Penicillin and rifampin ,100% were resist to tetracycline, 85.7% ampicillin,92.9% were resistant to chloramphenicol ,57.1% were resist to ciprofloxacin,21.4% were resist to gentamicin and 71.4% were resist to vavcomycin.
2. All samples were cultured under sterile conditions on Modified Edward,s medium which is a selective medium for isolation of Enterococcus.
3. Suspected Enterococcus colonies were identified biochemically and 14 isolates were identified as Enterococcus.
4. Biochemically identified Enterococci isolates were confirmed by PCR .
5. PCR was performed to identify E.faecalis and E.faecium by using specific primer for each. Six Isolates were E. faecalis and eight isolates were E. faecium .
6. All isolates were tested for antibiotic sensitivity test; Results revealed that 14.3 % of isolates were sensitive to penicillin G , 57.2 % of the isolates were
sensitive to gentamicin, 7.1% were sensitive to chloramphenicol, 21.4% were sensitive to vancomycin and 28.5 % were sensitive to ciprofloxacin.
On the other hand, 78.6 % of isolates were resistant to erythromycin, Penicillin and rifampin ,100% were resist to tetracycline, 85.7% ampicillin,92.9% were resistant to chloramphenicol ,57.1% were resist to ciprofloxacin,21.4% were resist to gentamicin and 71.4% were resist to vavcomycin.
Other data
| Title | Isolation and identification of Enterococcus spp from chicken | Other Titles | عزل وتصنيف سلالات الانتيروكوكاس فى الدجاج | Authors | Shimaa Hamdy Okasha Ahmed | Issue Date | 2017 |
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