DETERMINATION OF RELATIVE FERTILITY OF MALE DESERT ANIMALS USING MOLECULAR GENETIC TECHNIQUES
HEBA MOHAMED NOUR ELDIN SAAD;
Abstract
Heba Mohamed Nour Eldin Saad: Determination of Relative Fertility of Male Desert Animals Using Molecular Genetic Techniques. Unpublished M.Sc. Thesis, Department of Genetics, Faculty of Agriculture, In Shams University, 2017.
Camels are unique animals in many aspects and cannot be compared with other farm animals in their physiological responses and adaptation to arid environments. the Arabian camel is a better provider of food than cattle and sheep, which are severely affected by the heat, scarcity of water and forage. The reproductive efficiency of camels under natural conditions is generally regarded to be low. This is probably due to the relatively short breeding season, a longer prepubertal period. Animal fertility is a measure of reproductive success. Due to the complexity of the fertilization process, a single semen evaluation test is not enough to predict fertility. The development of molecular biology techniques during the past decades created exciting new means for studying livestock genetics and animal breeding. However, genetic studies on camels are scarce. Dromedary populations are genetically differentiated is poorly documented. fluorescent differential display is a rapid, specific, nonradioactive method in which fluorescently differentially labeled anchored oligo(dT) downstream primers are used in the reaction, with subsequent analysis of fluorescently labeled PCR products on an automated sequencer. Complete gene expression profiles, containing multiple mRNA fingerprints are possible by the simultaneous comparison of the multicolored banding patterns of the fluorescently differentially labeled products from several primer combinations. This modification of the differential display technique simplifies the assay and increases the throughput of high sample volumes required for comparative gene expression studies in various clinical applications (FDD) technology offers an unprecedented accuracy, sensitivity, and throughput in comprehensive and quantitative analysis of gene expression. These major improvements will allow
Camels are unique animals in many aspects and cannot be compared with other farm animals in their physiological responses and adaptation to arid environments. the Arabian camel is a better provider of food than cattle and sheep, which are severely affected by the heat, scarcity of water and forage. The reproductive efficiency of camels under natural conditions is generally regarded to be low. This is probably due to the relatively short breeding season, a longer prepubertal period. Animal fertility is a measure of reproductive success. Due to the complexity of the fertilization process, a single semen evaluation test is not enough to predict fertility. The development of molecular biology techniques during the past decades created exciting new means for studying livestock genetics and animal breeding. However, genetic studies on camels are scarce. Dromedary populations are genetically differentiated is poorly documented. fluorescent differential display is a rapid, specific, nonradioactive method in which fluorescently differentially labeled anchored oligo(dT) downstream primers are used in the reaction, with subsequent analysis of fluorescently labeled PCR products on an automated sequencer. Complete gene expression profiles, containing multiple mRNA fingerprints are possible by the simultaneous comparison of the multicolored banding patterns of the fluorescently differentially labeled products from several primer combinations. This modification of the differential display technique simplifies the assay and increases the throughput of high sample volumes required for comparative gene expression studies in various clinical applications (FDD) technology offers an unprecedented accuracy, sensitivity, and throughput in comprehensive and quantitative analysis of gene expression. These major improvements will allow
Other data
| Title | DETERMINATION OF RELATIVE FERTILITY OF MALE DESERT ANIMALS USING MOLECULAR GENETIC TECHNIQUES | Other Titles | تقديرالخصوبة النسبية لذكور الحيوانات الصحراوية باستخدام الدلائل الوراثيه الجزيئيه | Authors | HEBA MOHAMED NOUR ELDIN SAAD | Issue Date | 2017 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.