ROLE OF RETROTRANSPOSONS IN GENOMIC DIVERSITY IN DIFFERENT ORGANISMS
MARWA MAHMOUD SHEHATA AHMED;
Abstract
This study was carried out in the laboratories of the Genetics Dept., Fac. of Agric., Ain Shams Univ. during the period from 2008 to 2014. Biodiversity refers to variation within the living world, while genetic diversity represents the heritable variation within and between populations of organisms.
Transposable elements (TEs) are DNA elements that can transpose from one place in DNA to another. There are two main classes of TEs; DNA transposons; which act through a DNA intermediate, move from site-to-site through a cut-and-paste mechanism and multiply by using the host cell’s replication machinery, and retroelements (retrotransposons); which act through an RNA intermediate, move from site-to-site through a copy-and-paste mechanism.
Retrotransposons proved to be useful for molecular genetic studies and play an important role in genome evolution. five molecular genetic techniques (RAPD, ISSRs, IRAP, REMAP and RRAP) are used to study the role of retrotransposons in genomic diversity in different eukaryotic organisms such as lowering-eukaryotic single-cell organisms (yeast) and higher-flowering-plants (barley). The ojectives were:-
1. To study the effectiveness of the retrotransposon-based techniques (IRAP & REMAP) to clarify genomic diversity.
2. To evaluate the efficiency of new developed RRAP technique in detection of genetic diversity and in comparison with other retrotransposon-based techniques (IRAP & REMAP).
3. To compare the retrotransposon-based techniques (IRAP & REMAP) with the traditional molecular techniques such as RAPD & ISSRs-PCR.
Five yeast strains (UQM 49, NRRL Y-17008, LBC 1208, LBC 254 and ATCC 58523) and six barley cultivars (Giza 123, Giza 126, Giza 129, Giza 130, Giza 131 and Giza 2000) were used as DNA sources to assess the genetic diversity among yeast strains and barley cultivars.
Two retrotransposon-based primers (ScM1 and ScM2) were designed using ten Ty1-LTR-retrotransposon sequences (YBLWTy1-1, YBRWTy1-2, YDRCTy1-1, YDRCTy1-2, YDRWTy1-4, YDRWTy1-5, YERCTy1-1, YERCTy1-2, YGRCTy1-2 and YGRCTy1-3) and three freeware (Clustal X, CLC Free Workbench and PerlPrimer) and one commercial software (Lasergene7-PrimerSelect).
Transposable elements (TEs) are DNA elements that can transpose from one place in DNA to another. There are two main classes of TEs; DNA transposons; which act through a DNA intermediate, move from site-to-site through a cut-and-paste mechanism and multiply by using the host cell’s replication machinery, and retroelements (retrotransposons); which act through an RNA intermediate, move from site-to-site through a copy-and-paste mechanism.
Retrotransposons proved to be useful for molecular genetic studies and play an important role in genome evolution. five molecular genetic techniques (RAPD, ISSRs, IRAP, REMAP and RRAP) are used to study the role of retrotransposons in genomic diversity in different eukaryotic organisms such as lowering-eukaryotic single-cell organisms (yeast) and higher-flowering-plants (barley). The ojectives were:-
1. To study the effectiveness of the retrotransposon-based techniques (IRAP & REMAP) to clarify genomic diversity.
2. To evaluate the efficiency of new developed RRAP technique in detection of genetic diversity and in comparison with other retrotransposon-based techniques (IRAP & REMAP).
3. To compare the retrotransposon-based techniques (IRAP & REMAP) with the traditional molecular techniques such as RAPD & ISSRs-PCR.
Five yeast strains (UQM 49, NRRL Y-17008, LBC 1208, LBC 254 and ATCC 58523) and six barley cultivars (Giza 123, Giza 126, Giza 129, Giza 130, Giza 131 and Giza 2000) were used as DNA sources to assess the genetic diversity among yeast strains and barley cultivars.
Two retrotransposon-based primers (ScM1 and ScM2) were designed using ten Ty1-LTR-retrotransposon sequences (YBLWTy1-1, YBRWTy1-2, YDRCTy1-1, YDRCTy1-2, YDRWTy1-4, YDRWTy1-5, YERCTy1-1, YERCTy1-2, YGRCTy1-2 and YGRCTy1-3) and three freeware (Clustal X, CLC Free Workbench and PerlPrimer) and one commercial software (Lasergene7-PrimerSelect).
Other data
| Title | ROLE OF RETROTRANSPOSONS IN GENOMIC DIVERSITY IN DIFFERENT ORGANISMS | Other Titles | دور العناصر الوراثية المتنقلة الرجعية في التنوع الجينومي في كائنات مختلفة | Authors | MARWA MAHMOUD SHEHATA AHMED | Issue Date | 2014 |
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