Evaluation of Minichromosome maintenance complex component 5 (MCM5) and Forkhead box M (FOXM) genes expression in primary breast cancer
Abstract
SUMMARY
T
his study was done at Medical Biochemistry Department, Faculty of Medicine, Ain Sham University during the period from June 2013 – Januarey 2014 and included 54 patients and 20 normal volunteers.
The aim of the current study was to evaluate the expression of FOXM1 and MCM5 mRNA in malignant, benign breast lesions and healthy normal individuals after breast reduction surgery by semiquantitaive RT-PCR and to correlate the expression of both genes with the various clinico-pathological parameters in an attempt to evaluate the role of FOXM1 and MCM5 in tumor assessment and to explore their synergistic expression and their use as potential selected diagnostic marker in breast samples.
The studied individuals were classified into three main groups:
Group1, including 20 Healthy normal individuals after breast reduction surgery median age was 49.5 years
Group 2, including17 Benign breast lesion patients median age was 52 years
Group 3, including 37 Malignant breast cancer patient age median was 55 years.
All patients in the present study were subjected to complete detailed history taking, general and local examination, routine laboratory investigations, radio diagnostic investigations as chest X ray, and CT scan. Also all patients were subjected to detection of ER, PR Her-2 neu screening by immunohistochemistry in breast tissue samplesies.
The breast tumor was resected and sent for pathological staging and grading of the tumor according to (TNM) classification. Evaluation of FOXM1 and MCM5 mRNA expression in breast tissue was performed by Semiquantitative RT-PCR in relation to beta- actin as the housekeeping genes in all samples.
Then the results were calculated and statistically analyzed by the SPS software.
A significant difference was observed in the Positivity rate of MCM5 and FOXM1 RNA in breast tissue samples of the malignant group i.e. (78.3%, 70.2%) as compared with both benign group (47%,29.4%) and healthy normal group (5%,5%) respectively (P= 0.000).
Applying (0.865, 0.99), as a cut off value; which was calculated by ROC curve, the MCM5 and FOXM1 RNA showed (78.3%, 70.2%) sensitivity, (81%, 91.8%) specificity, (80.5%, 89.6%) PPV, (78.9%, 75.5%) NPV and (79.7%, 81%) accuracy.
T
his study was done at Medical Biochemistry Department, Faculty of Medicine, Ain Sham University during the period from June 2013 – Januarey 2014 and included 54 patients and 20 normal volunteers.
The aim of the current study was to evaluate the expression of FOXM1 and MCM5 mRNA in malignant, benign breast lesions and healthy normal individuals after breast reduction surgery by semiquantitaive RT-PCR and to correlate the expression of both genes with the various clinico-pathological parameters in an attempt to evaluate the role of FOXM1 and MCM5 in tumor assessment and to explore their synergistic expression and their use as potential selected diagnostic marker in breast samples.
The studied individuals were classified into three main groups:
Group1, including 20 Healthy normal individuals after breast reduction surgery median age was 49.5 years
Group 2, including17 Benign breast lesion patients median age was 52 years
Group 3, including 37 Malignant breast cancer patient age median was 55 years.
All patients in the present study were subjected to complete detailed history taking, general and local examination, routine laboratory investigations, radio diagnostic investigations as chest X ray, and CT scan. Also all patients were subjected to detection of ER, PR Her-2 neu screening by immunohistochemistry in breast tissue samplesies.
The breast tumor was resected and sent for pathological staging and grading of the tumor according to (TNM) classification. Evaluation of FOXM1 and MCM5 mRNA expression in breast tissue was performed by Semiquantitative RT-PCR in relation to beta- actin as the housekeeping genes in all samples.
Then the results were calculated and statistically analyzed by the SPS software.
A significant difference was observed in the Positivity rate of MCM5 and FOXM1 RNA in breast tissue samples of the malignant group i.e. (78.3%, 70.2%) as compared with both benign group (47%,29.4%) and healthy normal group (5%,5%) respectively (P= 0.000).
Applying (0.865, 0.99), as a cut off value; which was calculated by ROC curve, the MCM5 and FOXM1 RNA showed (78.3%, 70.2%) sensitivity, (81%, 91.8%) specificity, (80.5%, 89.6%) PPV, (78.9%, 75.5%) NPV and (79.7%, 81%) accuracy.
Other data
| Title | Evaluation of Minichromosome maintenance complex component 5 (MCM5) and Forkhead box M (FOXM) genes expression in primary breast cancer | Other Titles | تقييم جيني ام سي ام 5 و فوكس ام في مرضي سرطان الثدي | Issue Date | 2014 |
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