CHARACTERIZATION OF SOME VIRULENCE FACTORS OF HELICOBACTER PYLORI

Abstract

H. pylori is a gram negative micoaerophilic bacterium that appeared to play a role in peptic ulcer disease and it was considered as a risk factor for the development of gastric carcinoma. One of its virulence factors which is known to differ among H. pylori strains is the expression of 87-94 KDa vaculating cytotoxin.

This study aimed to characteriz some H. pylori virulence factors and it included 25 patients suffering from dyspeptic symptoms, their endoscopic examination showed signs of gastroduodenal disease (group f) and I 0 patients suffered from the same symptoms but without any endoscopic pathologic finding were selected as control group (group II).

To all of them the followings were done:

1- Full medical history taking.

2- Isolation of H. pylori from different clinical cases.

3- Identification of H. pylori.

4- Separation of H. pylori products that may contain its toxin.

5- Study of the cytopathic effect of H. pylori and its products that may contain toxin on different cell lines. 6- Characterization of H. pylori products by electrophoresis.

This study showed that:

1- H. pylori infection was higher in elderly patients (70%).

2- H. pylori infection was significantly higher in males (75%) than in females (20%). 3- H. pylori infection was significantly higher in patients with low socioeconomic status (78.9%) than in high socioeconomic status patients (16.7%).