“Antiviral Activity and Mode of Action of Extracted Proteins from Milk against Enteric Viruses”

Abstract

impact. Rotaviruses have been recognized as the major agents of diarrhea in infants and young children in developed as well as developing countries. In Egypt, diarrhea is one of the principal causes of death, mainly in the infant population. The antiviral activity of the cell-free supernatants (CFS) containing the metabolites of 6 yogurts and bio-yogurts made from bovine, buffalo and camel fermented milk under anaerobic conditions with bacterial starter, was initially evaluated against human Rotavirus using MTT colormeteric method, In addition to, determine the mode of action of antiviral activity. Then, the effect of pre-heat treatment of milk on antiviral activity of CFS of each yogurt was evaluated. All the CFS of yogurt was exhibited high antiviral activity against Rotavirus. Results obtained demonstrated that all CFS of yogurt can inhibit the replication of rotavirus and able to bind the viral particles to susceptible cells with early phases of rotavirus infection. On the other hand, pre-heat treatment induced losing the antiviral activity of yogurt metabolites with increasing temperature and time of heating. Generally, CFS of the yogurt containing metabolites fermented with probiotic bacteria showed high potential to be used for developing fermented milk-based foods or drugs. Key words: Rotavirus, Yogurt, Bovine milk, Buffalo milk and Camel Milk.