Evaluation of an Experimental Nano-Hydroxyapatite Material as a Root Perforation Repair Material
Sammar Mohammed Salah El-Din El-Sherif;
Abstract
The aim of the present study was to evaluate an experimental nano hydroxyapatite with and without silver nanoparticles as a root perforation repair material, with regards to:
• Synthesis and characterization of the materials.
• Physical properties including: setting time, dimensional changes & radiopacity.
• Sealing ability
• Biocompatibility and mineralization activity.
• Anti bacterial activity
Nano hydroxyapatite and nano hydroxyapatite with silver nanoparticles were prepared and characterized using (XRD) & (TEM).
Setting time was evaluated using Gilmore needle, dimensional change was evaluated using a digital caliper and the radiopacity was evaluated using aluminium step wedge.
Sealing ability was evaluated using dye extraction method.
For the evaluation of biocompatibility and mineralization activity; twenty-one rats were divided into 3 groups of 7 rats each according to the observation period of rats (1 week, 30 days, 60 days). Microscopic evaluation was made through a light microscope at X200 magnification.
Evaluation of antibacterial effect:
o Microorganisms: Enterococcus faecalis and Staphylococcus aureus.
o Evaluation methods: a. Agar diffusion method. b. Direct contact test.
Results:
I. Physical properties:
A. Setting time: there was a statistically significant difference between group III (MTA) 17.00 ± 1.00 minutes and each of group I (nano hydroxyapatite) 11.42 ± 0.97 minutes and group II (nano hydroxyapatite with nano silver) 11.57 ± 0.97 minutes.
B. Dimensional change: there was a statistically significant difference between group III (+ 0.13 ± 0.005% ----expansion) and each of group I (- 0.94 ± -0.01% --- shrinkage) and group II (-0.95 ± -0.05% ---shrinkage).
C. Radiopacity: the highest mean value of radiopacity was found in MTA 3.30 ± 0.29 mm of Al, followed by nano hydroxyapatite with nano silver 2.85 ± 0.45 mm of Al while the lowest mean value of radiopacity was found in nano hydroxyapatite 1.45 ± 0.53 mm of Al.
II. Sealing ability:
For group I (nano hydroxyapatite); group II (nano hydroxyapatite with nano silver) & group III (MTA): the lowest sealing ability was found in (1 day) while the highest sealing ability; was found in (3 months). In group I, II:
• Synthesis and characterization of the materials.
• Physical properties including: setting time, dimensional changes & radiopacity.
• Sealing ability
• Biocompatibility and mineralization activity.
• Anti bacterial activity
Nano hydroxyapatite and nano hydroxyapatite with silver nanoparticles were prepared and characterized using (XRD) & (TEM).
Setting time was evaluated using Gilmore needle, dimensional change was evaluated using a digital caliper and the radiopacity was evaluated using aluminium step wedge.
Sealing ability was evaluated using dye extraction method.
For the evaluation of biocompatibility and mineralization activity; twenty-one rats were divided into 3 groups of 7 rats each according to the observation period of rats (1 week, 30 days, 60 days). Microscopic evaluation was made through a light microscope at X200 magnification.
Evaluation of antibacterial effect:
o Microorganisms: Enterococcus faecalis and Staphylococcus aureus.
o Evaluation methods: a. Agar diffusion method. b. Direct contact test.
Results:
I. Physical properties:
A. Setting time: there was a statistically significant difference between group III (MTA) 17.00 ± 1.00 minutes and each of group I (nano hydroxyapatite) 11.42 ± 0.97 minutes and group II (nano hydroxyapatite with nano silver) 11.57 ± 0.97 minutes.
B. Dimensional change: there was a statistically significant difference between group III (+ 0.13 ± 0.005% ----expansion) and each of group I (- 0.94 ± -0.01% --- shrinkage) and group II (-0.95 ± -0.05% ---shrinkage).
C. Radiopacity: the highest mean value of radiopacity was found in MTA 3.30 ± 0.29 mm of Al, followed by nano hydroxyapatite with nano silver 2.85 ± 0.45 mm of Al while the lowest mean value of radiopacity was found in nano hydroxyapatite 1.45 ± 0.53 mm of Al.
II. Sealing ability:
For group I (nano hydroxyapatite); group II (nano hydroxyapatite with nano silver) & group III (MTA): the lowest sealing ability was found in (1 day) while the highest sealing ability; was found in (3 months). In group I, II:
Other data
| Title | Evaluation of an Experimental Nano-Hydroxyapatite Material as a Root Perforation Repair Material | Authors | Sammar Mohammed Salah El-Din El-Sherif | Issue Date | 2018 |
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