IN VITRO AND IN VIVO PROPGATION OF PASSION FRUIT
MARWA MOHAMED ABDELALL ABDELRAHMAN;
Abstract
ABSTRACT
The experiments had done at nursery of pomology department, Fac. Agric., Cairo Univ. and Tissue Culture laboratory& Germplasm preservation belonging to Hort. Res. Institute, Giza; during the period of 2013 to 2015. The aim of this work was to present a suitable protocol for propagation of passion fruit (Passiflora edulis) through tissue culture technique by direct organogenesis, and using the seed as a classic method, also using the seedlings as explants in micro propagation of passion fruit. The results indicated that the best significant sterilization treatment was Clorox at 20% + antibiotic (Amox) -15min that gave the lowest significant contamination 33.3% and the highest significant aseptic 77.7% by using the stem nod explant. Where it gave the highest significant viability 100% and lowest dead explant zero%. MS + 100ppm/l GA3 gave 100% seed germination and the best vegetative growth that gave 9.5cm. MS supplements with 0.1mg/l BAP+ 0.2mg/l NAA +2.2mg/l kin + 1g/l Casein hydrolyzed +2g/l NH4NO3 gave the highest significant parameters of passion multiplication,viz., 100% emergence vegetative growth length (3.812cm), 5 leaf number, 2 shoot number 1.7cm shoot length and healthy plantlets . Also MS supplements with 0.2mg/l NAA (sup culture media) that gave the highest significant parameters of passion multiplication, 100% emergence, 4.66cm vegetative growth length, 5 leaf number, 3 shoot number, 0.93cm shoot length. MS with 5mg/l IBA+ 5mg/l NAA+ 1g/l Casein hydrolyzed + 2g/l NH4NO3 gave the best significant results in the number of the main roots (7root/shoot), 3cm root system length, 1cm root system diameter, and color root yellow white, leaf color was glass green
a) The seeds sterilized by 20% Clorox + Amox. Antibiotic for 15 min., where it gave 100% alive explants, 22.2% contamination and 77.22% alive aseptic explants.
b) The best significant media of seeds was MS + 100ppm/l GA3 gave 100% seed germination and the best vegetative growth that gave 9.5cm.
c) The lowest significant ½MS that gave 15% seed germination and lowest vegetative growth length 3.3cm.
The agriculture mixtures of one part of peatmose and three part of sand (1:3 volume ratio) achieved the best results of number of leave/plantlet (14), leaf area 4.3cm², root number (18), success 50%, root width 7cm, root length 10cm of vegetative growth length 55cm, shoot number 1 for the plantlets and gave 95%success, 2shoots, 38cm length of vegetative growth, 17 leave/seedy plant, 10cm² leaf area, 14 roots/seedy plant, 16cm root length and 5cm root width for the seedy plants
Key word: Passion fruit, Micropropagation, seedy propagation
DEDICATION
The experiments had done at nursery of pomology department, Fac. Agric., Cairo Univ. and Tissue Culture laboratory& Germplasm preservation belonging to Hort. Res. Institute, Giza; during the period of 2013 to 2015. The aim of this work was to present a suitable protocol for propagation of passion fruit (Passiflora edulis) through tissue culture technique by direct organogenesis, and using the seed as a classic method, also using the seedlings as explants in micro propagation of passion fruit. The results indicated that the best significant sterilization treatment was Clorox at 20% + antibiotic (Amox) -15min that gave the lowest significant contamination 33.3% and the highest significant aseptic 77.7% by using the stem nod explant. Where it gave the highest significant viability 100% and lowest dead explant zero%. MS + 100ppm/l GA3 gave 100% seed germination and the best vegetative growth that gave 9.5cm. MS supplements with 0.1mg/l BAP+ 0.2mg/l NAA +2.2mg/l kin + 1g/l Casein hydrolyzed +2g/l NH4NO3 gave the highest significant parameters of passion multiplication,viz., 100% emergence vegetative growth length (3.812cm), 5 leaf number, 2 shoot number 1.7cm shoot length and healthy plantlets . Also MS supplements with 0.2mg/l NAA (sup culture media) that gave the highest significant parameters of passion multiplication, 100% emergence, 4.66cm vegetative growth length, 5 leaf number, 3 shoot number, 0.93cm shoot length. MS with 5mg/l IBA+ 5mg/l NAA+ 1g/l Casein hydrolyzed + 2g/l NH4NO3 gave the best significant results in the number of the main roots (7root/shoot), 3cm root system length, 1cm root system diameter, and color root yellow white, leaf color was glass green
a) The seeds sterilized by 20% Clorox + Amox. Antibiotic for 15 min., where it gave 100% alive explants, 22.2% contamination and 77.22% alive aseptic explants.
b) The best significant media of seeds was MS + 100ppm/l GA3 gave 100% seed germination and the best vegetative growth that gave 9.5cm.
c) The lowest significant ½MS that gave 15% seed germination and lowest vegetative growth length 3.3cm.
The agriculture mixtures of one part of peatmose and three part of sand (1:3 volume ratio) achieved the best results of number of leave/plantlet (14), leaf area 4.3cm², root number (18), success 50%, root width 7cm, root length 10cm of vegetative growth length 55cm, shoot number 1 for the plantlets and gave 95%success, 2shoots, 38cm length of vegetative growth, 17 leave/seedy plant, 10cm² leaf area, 14 roots/seedy plant, 16cm root length and 5cm root width for the seedy plants
Key word: Passion fruit, Micropropagation, seedy propagation
DEDICATION
Other data
| Title | IN VITRO AND IN VIVO PROPGATION OF PASSION FRUIT | Authors | MARWA MOHAMED ABDELALL ABDELRAHMAN | Issue Date | 2016 |
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