IMPLEMENTATION OF BIOTECHNOLOGY FOR IMPROVING SALT STRESS TOLERANCE IN PLANT USING PRROTEOMIC STUDY

Abstract

A proteomic approach was used to identify proteins affected by salt in the halophyte plant Atriplex nummularia L. A total of twenty seven protein spots were reproducibly detected and analyzed on 2 DE gel. These proteins showed changes under salinity condition in Atriplex leaves and were up-regulated. The identified protein spots were involved in biosynthesis, ATP generation, metabolism and photosynthesis. In an attempt to explore the data of the proteomic experiment, we have studied the effect of some compounds on wheat plant under salinity condition. The relevant of such compounds have been used as exogenous application under salinity. The selected compounds were proline, ascorbic acid, arginine, glutathione and glutamine. The results obtained from this experiment revealed that growth parameters (shoot length, root weight and grain weight), biochemical compounds (chlorophyll,